Effect of thyme and ginger metanoic extraction on the oxidative stability of sunflower oil

Document Type : Research Paper

Authors

1 Assistant Professor National University of Tabriz

2 university of tabriz

Abstract

Due to unsaturated fatty acids of GSO, is exposed to various types of chemical reactions, including enzymatic reactions and lipid oxidation. In this regard, the use of antioxidants for increasment the resistance of the oil to oxidation, is essential. Due to the poor nutritional and cancerous effects of these compounds and the consumer's desire to use natural compounds, the use of natural antioxidants is considered by researchers instead of industrial antioxidants. Spices (ginger, pepper, cinnamon, cardamom, thyme) and aromatic vegetables such as basil and peppermint with essential oils (EOS) containing multiple polyphenols with anti-oxidant and anti-microbial properties are a good source of natrul antioxidant (Teixeira et al., 2013; Srinivasan, 2017). Ginger is an important medicinal herb and has several properties, including anti-nausea, cardiovascular, antibacterial, liver anti-inflammatory, lowering blood cholesterol stimulates brain circulation and stimulates digestion. The presence of antioxidants in ginger causes the removment of free radicals from oil. Thyme is the other valuable herbs with high antioxidant and antimicrobial activity and is one of the world's best herbs in terms of high levels of antioxidants. The phenolic antioxidants in thyme, eliminate free radicals and prevent oxidative stress in all body systems. In spite of studies on the use of phenolic compounds in oxidation stability of oils, the use of ginger and thyme EOS in combination, as a natural antioxidant has not been investigated for the stability of grape seed oil. By extracting these phenolic compounds and adding to grape seed oil, it can be expected that the oleoresin in these plant extracts, in comparison to industrial antioxidants, increases the oxidative stability of grape seed oil.
Methods
Extraction of phenolic compounds from ginger and thyme
25 g of powdered ginger or thyme was placed in a cellulose ring of suksele apparatus. Then their phenolic compounds were extracted using methanol solvent. Extraction was done at a temperature higher than boiling point of solvent (65 °C) and continued until extraction complete. The solvent was removed using a rotary evaporator and the extracted compounds was stored for further experiments in the refrigerator (Seidi Damyeh and Niakousari, 2015). Different types and concentrations of metanoic extraction were added to GSO (Table 1) and experiments was done during 45 days of storage.

Table 1
Number Timar
1 Control sample (grape seed oil without antioxidant)
2 GSO+ α-tocopherol (200 ppm)
3 GSO+ ginger phenolic compounds (300 ppm)
4 GSO+ thyme phenolic compounds (300 ppm)
5 GSO+(ginger+thyme) phenolic compounds (300 ppm)

Experiments
The properties of sunflower oil including acidity, peroxide and tiobarbioturic acid (TBA) indices, total phenol and antioxidant activity were studied during 45 days of storage.
Statistical Analysis
The results were analyzed using completely randomized blocks during 45 days of the storage time. One-way ANOVA and Duncan’s mean comparison tests were used at 5% significance (p < 0.05).
Results and discussions
Acidity
acidity of the all samples increased significantly during the 45 days of storage (p < 0.05). The sample contained 300 ppm of (thyme and ginger) phenolic compounds on the 45th day had the lowest acidity and the highest acidity was observed in the sample without antioxidants (control sample). GSO containing two species of thyme and ginger had fewer amounts of FFA and were able to prevent oxidation, resulting in less acidity,

Peroxide index
The rate of peroxide index increased significantly during the 45 days of storage. In all days, the control sample had the highest amount of peroxide because the presence of antioxidants in different samples caused the peroxide to be neutralized and consequently reduced its amount and against the lowest index after 45 days of the sample.
TBA index
In all samples, the level of TBA index increased significantly, while the lowest amount of the index after 45 days of storage was related to the sample containing α-tocopherol and the sample containing 300 ppm of thyme metanoic extraction. It should also be noted that no significant difference was found between them. In the case of other treatments, it can be stated that the highest level of this index is related to the control sample without any antioxidants. TBA index shows the amount of secondary oxidation products, especially aldehydes.
Total phenol
Over time, Total phenol (mg of gallic acid per liter) of oil samples containing different concentrations of antioxidants has been significantly reduced in all samples. According to the results, it can be stated that with increasing concentrations of phenolic compounds in oil samples, the total phenol content also increased. This can be attributed to the presence of phenolic compounds in both essential oils of thyme and ginger. Phenolic compounds play an essential role in antioxidant activity due to the presence of hydroxyl groups in their structure and their ability to donate hydrogen to the free radicals.
Conclusion
The results showed acidity of the samples increased during storage. Peroxide value and TBA of sunflower oil samples were significantly increased during storage. The lowest amount of these indices after 45 days was related to the samples containing α-tocopherol and 300 ppm mixture of ginger and thyme, respectively. The total phenol also was increased by increasing the concentration of extract which can be due to the presence of phenolic compounds in the ginger and thyme extracts. The radical scavenging activity of all samples was decreased during storage. The highest antioxidant activity was related to the samples containing α-tocopherol and then those containing 300 ppm of ginger +thyme extract In general, it can be concluded that the metanoic extraction of ginger and thyme can be used as a natural antioxidants in the edible oils to prevent their oxidation.

Keywords


حسن فامیان ف و پزشکی نجف­آبادی الف، تولید نانوامولسیون حاوی لینولئیک اسیدکونژوگه (CLA) به روش تشکیل خود به خودی وغنی­سازی شیر کم­چرب پاستوریزه با آن، نشریه پژوهشهای صنایع غذایی، (4)27، 145-135.

مرندی الف، محمدی م، فتح الهی ع و پزشکی نجف­آبادی الف، تولید نانوامولسیون حاوی لینولئیک اسیدکونژوگه (CLA) به روش تشکیل خود به خودی وغنی­سازی شیر کم­چرب پاستوریزه با آن، نشریه پژوهشهای صنایع غذایی، (4)28، 167-157.

Alothman M, Bhat R & Karim AA, 2009. Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chemistry 115(3): 785-788.

Aluyor E O, & Ori-Jesu M, 2008. The use of antioxidants in vegetable oils–A review. African Journal of Biotechnology: 7(25).
Asensioa CM, Grossob NR, & Juliania HR, 2015. Quality preservation of organic cottage cheese using oregano essential oils. LWT - Food Science and Technology 60(2): 664-671.
Bayala B, Bassole IH, Scifo R, Gnoula C, Morel L, Lobaccaro JMA, & Simpore J, 2014. Anticancer activity of essential oils and their chemical components ‐ A review. American Journal of Cancer Research 4: 591– 607.
Bellik Y, Benabdesselam F, Ayad A, Dahmani Z, Boukraa L, Nemmar A, & Iguer-Ouada M, 2013. Antioxidant activity of the essential oil and oleoresin of Zingiber Officinale Roscoe as affected by chemical environment. International journal of Food Properties 16(6): 1304-1313.
Cakmakci S, Gundogdu E, Dagdemir E, & Erdogan U, 2014. Investigation of the Possible Use of Black Cumin (Nigella sativa L.) essential oil on butter stability. The Journal of the Faculty of Veterinary Medicine 20 (4): 533-539.
Cañizares-Macías MP, García-Mesa JA, & De Castro ML, 2004. Determination of the oxidative stability of olive oil, using focused-microwave energy to accelerate the oxidation process. Analytical and bioanalytical chemistry 378(2): 479-483.
Chang S, Bassiri A, & Jalali H, 2013. Evaluation of antioxidant activity of fennel (Foeniculum vulgare) seed extract on oxidative stability of olive oil. Journal of Chemical health risks 3(2): 53-61.
El Asbahani A, Jilale A, Voisin SN, HAddi EA, Casabianca H, Mousadik A, Hartmann DJ, & Renaud FNR, 2015. Chemical composition and antimicrobial activity of nine essential oils obtained by steam distillation of plants from the Souss‐Massa Region (Morocco). Journal of Essential Oil Research 27: 34–44.
Elizabeth I, Ifeanyi G, & Veronica O, 2013. Production of oleoresin from ginger (Zingiber officinale) peels and evaluation of its antimicrobial and antioxidative properties. African Journal of Microbiology Research 7(42): 4981-4989.
Garcıa JL, Luque de Castro MD, 2004. Ultrasound assisted soxhlet extraction, an expeditive approach for solid sample treatment, application to the extraction of total fat from oleaginous seeds. Journal of Chromatograph Analysis 1034: 237–42.
Hosseinipour SH, Peyghambari SY, & Rostamza H, 2012. Comparison of olive leaf extract and BHT antioxidant on the shelf life of Rainbow trout fish (Oncorhynchus mykiss) in cold storage at 4±1. Journal of Food Processing and Preservation 4 (2): 67-83.
Iqbal S, & Bhanger MI, 2007. Stabilization of sunflower oil by garlic extract during accelerated storage. Food Chemistry 100: 246-254.
Kizhakkayil J, & Sasikumar B, 2011). Diversity, characterization and utilization of ginger: A review. Plant Genetic Resources 9(3): 464-477.
Lahlou M, 2004. Methods to study the phytochemistry and bioactivity of essential oils. Phytother Research PTR 18: 435–448.
Liu Q, & Yao H, 2007. Antioxidant activities of barley seeds extracts. Food Chemistry 102(3): 732-737.
Mirzaei A, Mohammadi J, Mirzaei N, & Mirzaei M, 2011. The antioxidant capacities and total phenolic contents of some medicinal plants in Iran. Journal of Fasa University of Medicinal Sciences 1(3): 160-166.
Murthy PS, & Gautam R, 2015. Ginger oleoresin chemical composition, bioactivity and application as bio‐preservatives. Journal of Food Processing and Preservation39(6): 1905-1912.
Özkan G, & Özcan MM, 2017. Antioxidant activity of some medicinal plant extracts on oxidation of olive oil. Journal of Food Measurement and Characterization 11(2), 812-817.
Pourreza N, 2013. Phenolic compounds as potential antioxidant. Jundishapur Journal Pharmological Production 8(4): 149-50.

Rafei S, Azizkhani M, Areaei P, 2017. Impact of antioxidative properties of cumin and tarragon essential oils on the quality of full-fat white cheese. Food Technology & Nutrition 14(4): 79-90.

Roshan M, Esmaeel-zade Kenari R, 2017. Antioxidant effect of strawberry leave extracts on stabilization of sunflower oil during storage condition. Journal of Food Science and Technology 65(14): 301-308.
Sadeghi Z, Kuhestani K, Abdollahi V, & Mahmood A, 2014. Ethno-pharmacological studies of indigenous medicinal plants of Saravan region, Baluchistan. Iranian Journal of Ethnopharmacol 153: 111–118.
Said PP, Arya OP, Pradhan RC, Singh RS, & Rai BN, 2015. Separation of oleoresin from ginger rhizome powder using green processing technologies. Journal of Food Process Engineering 38(2):107-114.
Salariya AM, & Habib F, 2003. Antioxidant activity of ginger extract in sunflower oil. Journal of Food Science and Agriculture 83(7): 624-629.

Salmanian S, Sadeghi Mahoonak AR, Alami M, Ghorbani M, 2013. Antioxidant activity of hawthorn (Crataegus elbursensis) extract on stability of soybean oil. Journal of Food Research 23(2): 199-209.

Sefidkon F, Dabiri M, & Mirmostafa SA, 2004. The Composition of thymus serpyllum L. oil. Journal of Essentional Oil Research 16: 184-5.
Seidi Damyeh, M, & Niakousari M, 2016. Impact of ohmic‐assisted hydrodistillation on kinetics data, physicochemical and biological properties of Prangos ferulacea Lindle. Essential oil: Comparison with conventional hydrodistillation. Innovative Food Science Emerging Technology 33: 387–396.
Sharififar F, Moshafi MH, Mansouri SH, Khodashenas M, Khoshnoodi M, 2007. In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methaol extract of endemic Zataria multiflora. Boisystem Food Control 18: 800-5.
Singh S, & Singh RP, 2008. In vitro methods of assay of antioxidants: An overview. Food reviews international 24(4): 392-415.
Singleton VL, Rossi JA, 1965. Colorimetry of total phenolics with phosphor molybdic-phosphotungstic. American Journal of Enology and Viticulture 16:144-58.
Souri E, Amin G, Farsam H, & Barazandeh Tehrani M, 2008. Screening of antioxidant activity and phenolic content of 24 medicinal plant extracts. Daru 16(2): 83-87.
Srinivasan K, 2017. Ginger rhizomes (Zingiber officinale): A spice with multiple health beneficial potentials. PharmaNutrition 5(1): 18-28.
Sun L, Zhang J, Lu X, Zhang, L & Zhang, Y, 2011. Evaluation to the antioxidant activity of total flavonoids extract from persimmon (Diospyros kaki L.) leaves. Food and Chemical Toxicology 49(10): 2689-2696.
Teixeira B, Marques A, Ramos C, Neng NR, Nogueira JMF, Saraiva JA, & Nunes ML, 2013. Chemical composition and antibacterial and antioxidant properties of commercial essential oils. Indian Crops Products 43: 587–595.
Tominaga H, Kobayashi Y, Goto T, Kasemura K, Nomura M, 2005. DPPH radical scavenging effect of several phenyl propanoid compounds and their glycoside derivatives.Yakucaku Zasshi 125(4): 371-5.
Villanueva Bermejo D, Angelov I, Vicente G, Stateva RP, Rodriguez Garcia‐Risco M, Reglero G, Ibanez E, & Fornari T, 2015. Extraction of thymol from different varieties of thyme plants using green solvents. Journal of Food Science and Agriculture 95: 2901–2907.
Wang W, Jung J, Zhao Y, 2017. Chitosan-cellulose nanocrystal microencapsulation to improve encapsulation efficiency and stability of entrapped fruit anthocyanins. Carbohydrate Polymers 157: 1246–1253.
Zandinava F, 2017. Effect of ginger oleoresin on oxidative stabity of olive oil.MS. tesis, University of Tabriz, Tabriz, Iran.