Prediction of shelf life and correlation of quality properties peeled white shrimp (Metapenaeus affinis) during chilling

Document Type : Research Paper

Authors

Abstract

Introduction: Shrimp is one of the popular seafood consumed in the Iran. In Abadan (West Iran), Metapenaeus affinis, called as king prawn, are caught from marine water (Persian Gulf), processed and sold in the local market or exported. There is a reduction quality due to microbiological and enzymes activities during postmortem storage, which result in undesired odors and off-flavors over time, causing ultimate rejection by the consumer and making shrimp unfit for consumption. Specific spoilage organisms, a few members of the microbial community of freshly caught or processed seafood, produce various volatile compounds such as trimethylamine (TMA) and total volatile nitrogen bases (TVB-N), which could function as indicators of spoilage. Physicochemical indexes (pH, trimethylamine (TMA), total volatile nitrogen bases (TVB-N) and thiobarbitoric acid reactive substances (TBARS) are used to evaluate the freshness of fishery products. White shrimp (Metapenaeus affinis) is a warm-water speciesnative to the eastern Pacific coast from the Gulf of California, Mexico tonorthern Peru. The world aquaculture production of white shrimp was about 4.2 million tonnes with a global market value of USD24.4 billion in 2016. Currently, Pacific white shrimp is themost important species economically, and it is accounted for about 70%of the total shrimp production in the world due to its high nutrient content of amino acids, peptides, polyunsaturated fatty acids, and other useful substances. However, because shrimp is subject to natural contamination by many bacterial species and contain a large amount of non-protein nitrogenous compounds and autolytic enzymes, it is highly perishable and its post-mortem changes occur rapidly, which result in an obvious off-taste and soft texture. In general, sea-food microbiota originates from the skin or intestines of the processed objects, and contamination occurs during the successive steps of food processing. Shrimp spoilage is attributed mainly to the uncontrolled growth and subsequent various metabolic activities of microbiota. It is commonly assumed that only specific spoilage organisms (SSO) participate in the spoilage process, and they produce metabolites that result in off-odors and off-flavors. The identification of SSO that are responsible for spoilage re-quires sensory, microbiological and chemical studies. The growth of SSO results in the breakdown of macromolecules in shrimp, which causes the tissue of shrimp to lose its elasticity and produce off-odors. The modification of microbiota would lead to the change in pattern andprocess of spoilage in shrimp. Therefore, the aim of the present assay is to investigate of different temperatures (ice and refrigerator) on peeled shrimp Metapenaeus affinis.
Material and methods: Freshly caught shrimp Metapenaeus affinis were collected from the Persian Gulf in Khozestan (south Iran). The average weight of shrimp was 15.26±0.05 g per shrimp. Immediately after collection, samples were cooled with ice and transported to the Department of Marine Natural Resources, Khorramshahr University of Marine Science and Technology within 1h. The ratio of shrimp to ice was 1:3 (w/w). Upon arrival, shrimp were peeled and washed in cold water. After draining, shrimp weighing approximately 200 g were packed in a zip bag and were stored at ice and refrigerator. Shrimp samples were kept in ice in a plastic container with drain holes. Shrimp were re-iced daily to maintain the same ratio. All samples were taken for microbiological, physicochemical, and sensory analyses every 4 days for up to 16 days. Average were compared through an Analysis of Variance (ANOVA) and effects were considered significant (by Duncan´s test) when p-value £0.05. Pearson correlation analysis with 95% of confidence interval was used to determine the relationship between time of iced storage and quality parameters.
Results and discussion: Variations in physicochemical index were observed throughout the storage period. Sensory analysis attributes exhibited significant variations and correlations close 0.800 with time storage, which is a showing of the shrimps´ loss of freshness. A regression analysis using the acceptability limit mesophilic counts (7 log cfu/g) yielded a shelf life for white shrimp stored on ice and refrigerator of 7 and 4 days respectively.
Conclusion: The TVBN, microbiological and sensory analysis displayed very strong correlations with storage time, and they may be considered suitable indicators for evaluating white shrimp spoilage stored on ice and refrigerator.

Broekaert K, Heyndrickx M, Herman L, Devlieghere F and Vlaemynck G, 2013. Molecular identification of the microbiota of peeled and unpeeled brown shrimp (Crangon crangon) during storage on ice and at 7.5 ºC. Food Microbiology 36: 123-134.
Chinivasagam HN, Bremner HA, Wood AF, Nottingham and SM. 1998. Volatile components associated with bacterial spoilage of tropical prawns. International Journal of Food Microbiology 42:45–55.
Chytiri S, Chouliara I, Savvaidis IN and Kontominas MG, 2004. Microbiological, chemical and sensory assessment of iced whole and filleted aquacultured rainbow trout. Food Microbiology 21: 157-165.
Dabade DS, den Besten HMW, Azokpota P, Nout MJR, Hounhouigan DJ and Zwietering MH, 2015. Spoilage evaluation, shelf-life prediction, and potential spoilage organisms of tropical brackish water shrimp (Penaeus notialis) at different storage temperatures. Food Microbiology 48: 8-16.
Goulas AE and Kontominas MG, 2005. Effect of salting and smoking-method on the keeping quality of chub mackerel (Scomber japonicus): Biochemical and sensory attributes. Food Chemistry 93: 511–520.
Gram L and Huss H, 1996. Microbiological spoilage of fish and fish products. Food Microbiology 33: 121-137.
Gram L and Dalgaard P, 2002. Fish spoilage bacteria-problems and solutions. Current Opinion in Biotechnology 13: 262-266.
Heu MS, Kim JS and Shahidi F, 2003. Components and nutritional quality of shrimp processing by-products. Food Chemistry 82: 235-242.
ICMSF. 1978. Sampling for microbiological analysis (2nd ed.). In microorganisms in foods, Vol. 2 Toronto, Canada: University of Toronto Press: The International Commission on Microbiological Specifications for Foods.
Joseph J and Iyer TSG, 2002. Sensory evaluation. In K. Gopakumar (Ed.), Textbook of fish processing and technology (pp. 445-467). New Delhi, India: Indian Council of Agricultural Research.
Kaewprachu P, Osako K, Benjakul S and Rawdkuen S, 2016. Effect of protein concentrations on the properties of fish myofibrillar protein based film compared with PVC film. Journal of Food Science and Technology 53: 2083-2091.
Liston J, 1980. Microbiology in fishery science. In: Connell, J.J. (Ed.), Advances in Fish Science and Technology. Fishing News Book Ltd, Farnham, Surrey, England, pp. 138e157.
Jay JM, 1986. Modern Food Microbiology, third ed. Van Nostrand Reinhold Company, New York.
Kirschnik PG and Viegas EMM, 2004. Alterações na qualidade do camarão de água doce Macrobrachium rosenbergii durante estocagem em gelo. Ciência e Tecnologia de Alimentos 23: 407-412.
Kilincceker O, Dogan IS and Kucukoner E, 2009. Effect of edible coatings on the quality of frozen fish fillets. LWT - Food Science and Technology 42: 868–873.
Lopez-Caballero M, Gonçalves A and Nunes M, 2002. Effect of CO2/O2-containing modified atmospheres on packed deepwater pink shrimp (Parapenaeus longirostris). European Food Research and Technology 214: 192-197.
Okpala COR, Choo WS and Dykes GA, 2014. Quality and shelf life assessment of Pacific white shrimp (Litopenaeus vannamei) freshly harvested and stored on ice. LWT-Food Science and Technology 55: 110-116.
Sallam KI, 2007. Antimicrobial and antioxidant effects of sodium acetate, sodium lactate, and sodium citrate in refrigerated sliced salmon. Food Control 18: 566–575.
Siripatrawan U and Noipha S, 2012. Active film from chitosan incorporating green tea extract for shelf life extension of pork sausages. Food Hydrocolloids 27: 102-108.
Suvanich V, Jahncke ML and Marshall DL, 2000. Changes selected chemical quality characteristics of channel catfish frame mince during chill and frozen storage. Food Science 65: 24-29.
Vieira RHSF, Vieira GHF, Rocha CAS, Saker-Sampaio S and Sampaio AH, 1990. Avaliação sensorial e química de lagosta do gênero Panulirus White, estocada em gelo. Arquivos de Ciências do Mar 28: 69-92.
Woyewoda AD, Shaw SJ, Ke PJ and Burns BG, 1986. Recommended laboratory methods for assessment of fish quality. Canadian Technical Report of Fish and Aquatic Science. 1448p.
Zeng QZ, Thorarinsdottir KA and Olafsdottir G, 2005. Quality changes of shrimp (Pandalus borealis) stored under different cooling conditions. Journal of Food Science 70: 459-466.