Evaluation of Antibacterial Activity and Chemical Composition Determination of Essential Oil of Mentha aquatica Collected from north of Iran

Document Type : Research Paper

Authors

1 Graduated student, Department of Food Science and technology, Azadshahr branch, Islamic Azad University, Azadshahr, Iran

2 Department of Microbiology, Azadshahr branch, Islamic Azad University, Azadshahr, Golestan province, Iran

Abstract

Introduction: The importance of herbal and spice essential oils is that in addition to creating flavor in foods, their main active ingredient has antimicrobial effects, for this reason, consumers prefer herbal oils to chemicals. The Lamiaceae are a family of flowering plants commonly known as the mint family. Many members of this family are aromatic herbs and are widely used as spices in the food industry. Mentha (also known as mint) is a genus of plants in the family Lamiaceae . The species that makes up the genus Mentha are widely distributed and can be found in many environments. Most grow best in wet environments and moist soils. The genus Mentha consists of over 20-30 species that grow widely throughout the world. Members of this genus are one of the most important plants producing essential oils. There are many varieties of essential oils in different species (Zargari, 1995). Mentha aquatica L. that known in the northern regions of Iran locally called Ojji, is a commonly spice herb has used. This plant grows in aquatic places throughout Iran, especially in northern Iran. One of the main habitats of this plant in Iran is Mazandaran province in northern Iran (Getahun et al., 2008). Food poisoning caused by Escherichia coli and Staphylococcus aureus are known as the most important causes of food poisoning. This study was caried out to evaluate the chemical composition and antibacterial activity of essential oil of Mentha aquatica L. against S. aureus and E. coli
Material and methods: Plant of Ojji (Mentha aquatic) was obtained from from local market in Sari township located in Mazandaran Province in northern Iran and was approved by the botany laboratory of Islamic Azad University, Gorgan branch. The essential oil of herb leafs was extracted by hydrodistillation method and Clevenger apparatus. Gas chromatography–mass spectrometry (GC-MS) was used to identify essential oil chemical compounds. The gas chromatograph used was Agilent 6890 with a capillary column of 30 m in length and an internal diameter of 250 μm and a layer thickness of 0.25 μm HP-5MS. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the essential oil of this plant oil were determined using micro-dilution broth method or turbidimetric assay. The Bacterial strains used in this study were two species of Escherichia coli PTCC 1338 and Staphylococcus aureus PTCC 1112. The bacteria were provided in lyophilized form from Biotechnology Institute in Iranian Research Organization for Sciences and Technology, Tehran. Different dilutions of essential oil in Mueller Hinton Broth were exposed with bacterial suspension of 5 ×105CFU/ml of each of the bacteria tested for 24 hours at 37 ° C. After this time the results were recorded as microbial turbidity of visible. The last dilution (lowest concentration) in which microbial turbidity was not observed, as the minimum inhibitory concentration (MIC) was considered. For the determination of MBC, from the tube that contained essential oil concentrations higher than the MIC were cultured onto the Nutrient agar medium. The MBC was defined as the lowest concentration that allowed no visible growth on the agar (Cockerill et al., 2012).
Results and discussion: The results of the gas chromatography analysis were determined 27 chemical compounds that formed more than 98% of the essential oil compounds. 3-Carene were the highest concentrations chemical compound of Mentha aquatica essential oil with 61.24%. Cineol, Limonen and Agarospirol were other known compounds of the essential oil of this plant that were measured in quantities of 9.4%, 6.81% and 4.78%, respectively. 3-Carene is a hydrocarbon monoterpene. Therefore, the antibacterial activity of the essential oil of Mentha aquatica can be attributed to the presence of hydrocarbon monoterpens. Identification of the chemical constituents of the essential oil of Mentha aquatica in different parts of the world indicates different reports of the presence of different compounds in this essential oil. Different findings may be due to intrinsic properties of essential oils such as pre-harvest factors such as variety, environmental conditions, ecological factors and differences in extraction methods. Although production of secondary metabolites in plants are made by genetic processes, they are affected by environmental factors. The results of antibacterial tests indicated that S. aureus was more sensitive than E. coli to the essential oil of this plant as MIC and MBC of essential oil of this herb on the S. aureus 1.56 and 1.56 mg/ml and on the E.coli was 3.12 and 6.25 mg/ml respectively. The cause of the lower sensitivity of gram-negative bacteria may be due to the presence of an outer membrane in gram-negative bacteria that restricts the release of hydrophobic components of the essential oil into the lipopolysaccharide layer. One of the important properties of the essential oils is their hydrophobic properties, which distribute them in lipid portions of the cell wall, altering and destroying their structure and increasing their permeability. As a result, much of the ions and other vital contents of the cell leak out, eventually leading to bacterial death. Concerning the antibacterial activity of the essential oils, it has been suggested that phenolic metabolites in plants such as Mentha aquatica are capable of releasing a hydrogen from the hydroxyl group present in their aromatic ring and causing the oxidation of free radicals in lipids and other cellular membrane biomolecules and its destruction and thus produce their antioxidant, antimicrobial and anti-inflammatory properties.
Conclusion: The antibacterial activity of essential oil of Mentha aquatica can be attributed to the presence hydrocarbon monoterpene compounds such as 3-Carene along with compounds such as Cineol, Limonen, Agarospirol, Eucalyptol and Menthone that leads the potential for its use as a natural preservative in food.

Keywords


Abrishamchi P, Khaje Karamadini M, Houshyar-Sarjami R, Zaker A, Asili J, Porsa H and Zarif R, 2016. Antibacterial effect of essential oils from Salvia leriifolia Benth against some oral pathogens. Journal of Microbial World 9(3): 215-225.
Adams RP, 2001. Identification of Essential Oils Components by Gas Chromatography/Quadrupole Mass Spectroscopy. Allured Publishing Corporation, Illinois, 469.
Aggarwal B, Sundaram C, Malani N and Ichikawa H, 2007. Curcumin: the india solid gold. Advance in Experimental Medicine and Biology 59: 31-38.
Agostini F, Dos Santos ACA, Rossato M, Pansera MR, Dos Santos PL, Serafini LA, Molon R and Moyna P, 2009. Essential Oil Yield and Composition of Lamiaceae Species Growing in Southern Brazil. Brazilian Archives of Biology and Technology 52: 473–478.
Andro AR, Boz I, Zamfirache MM and Burzo I, 2013. Chemical composition of essential oils from Mentha aquatica L. at different moments of the ontogenetic Cycle. Journal of Medicinal Plants Research 7(9): 470-473.
Ayfer D and Turgay O, 2003. Antimicrobil activities of various medicinal and commercial plant extracts. Turkish Journal of Biology 27: 157-62.
Burt S, 2004. Essential oils: their antibacterial properties and potential application in foods-a review. International Journal of Food Microbiology 94(3): 223-253.
Carson CF, Mee BJ and Riley TV, 2002. Mechanism of action of Melaleuca alternifolia oil on Staphylococcus aureus determined by time-kill, lysis, leakage and salt tolerance assays and electron microscopy. Antimicrobial Agents and Chemotherapy 46(6): 1914-1920.
Celiktas OY, Kocabas EH, Bedir E, Sukan FV, Ozek T and Baser KHC, 2007. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chemistry 14: 323-8.
Cockerill FR, Wikler MA, Alder J, Dudley MN, Eliopoulos GM, Ferraro MJ, Hardy DJ, Hecht DW, Hindler JA, Patel JB, Powell M, Swenson JM, Thomson RB, Traczewski MM, Turnidge JD, Weinstein MP and Zimmer BL 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard. CLSI document M07-A9.9. 9 ed. C.L.S.I. (Clinical and Laboratory Standard Institute), Pennsylvania, PA, USA.
Deans SG and Ritchi G, 1997. Antibacterial properties of plant essential oils. International journal of food microbiology 5: 165-180.
Dhifi W, Litaiem M, Jelali N, Hamdi N and Mnif W, 2011. Identification of A New Chemotye of the Plant Mentha aquatica Grown in Tunisia: Chemical Composition, Antioxidant and Biological activities of its Essential Oil. Journal of Essential Oil Bearing Plants 14(3): 320-328.
Esmaeili A, Rustaiyan A, Masoudi S and Nadji K, 2006. Composition of the Essential Oils of Mentha aquatica L. and Nepeta meyeri Benth. from Iran. Journal of Essential Oil Research 18(3): 263-265.
Fancello F, Zara S, Petretto GL, Chessa M, Addis R, Rourke JP and Pintore G, 2017. Essential oils from three species of Mentha harvested in Sardinia: chemical characterization and evaluation of their biological activity. International Journal of Food Properties 20(2): 1751-1761.
Ferreira FS, Davanad L, Luthria DL, Sasaki T and Heyerick A, 2010. Flavonids from Artemisia annua L. as Antioxidants and their potential synergism with artemisinin against malaria and cancer. Molecules 15: 3135-3170.
Gruenwald J, Brendler T, Jaenicke C, 2000. Physicians' Desk Reference (PDR) for Herbal Medicines pp.813-816.
Getahun Z, Asres K, Mazumder A and Bucar F, 2008. Essential Oil Composition, Antibacterial and Antioxidant Activities of Mentha aquatica Growing in Ethiopia. Ethiopian Pharmaceutical Journal 26: 9-16.
Gulluce M, Shain F, Sokmen M, Ozer H, Daferera D and Ozkan H, 2007. Antimicrobial and antioxidant properties of the essential oils and menthonal extract from mentha longifolial L.spp. Food chemistry 103: 1449-1456.
Heywood V, 2002. The conservation of genetic and chemical diversity in medicinal and aromatic plants. In: Sener, B. (Ed.), Biodiversity: Biomolecular Aspects of Biodiver sity and Innovative Utilization, Kluwer Academic/Plenum Publishers, New York. PP.13-22
Hussain AI, Anwar F, Sherazi STH and Przybylski R, 2008. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chemistry 108: 986-95.
Jerkovic I and Mastelic J, 2001. Composition of Free and Glycosidically Bound Volatiles of Mentha aquatica L. Croatica Chemica Acta CCACAA 74(2): 431-439.
Kazem Alvandi R, Sharifan A and Aghazadeh Meshghi M, 2011. Study of chemical composition and antimicrobial activity of peppermint essential oil. Journal of Comparative Pathobiology 7(4): 355-364.
Marotti M, Piccaglia R, Giovanelli E, Deans SG and Eaglesham E, 1994. Effects of planting time and mineral fertilization on peppermint (Mentha piperita L.) essential oil composition and its biological activity. Flavour and Fragrance Journal 9: 125-129.
Moreira MR, Ponce AG, Dell vella CE and Roura SI, 2005. Inhibitory parameters of essential oils to reduce a foodborne pathogen. LWT - Food Science and Technology 38(2005): 565-570.
Morteza-Semnani K, Saeedi M and Akbarzadeh M, 2006. The Essential Oil Composition of Mentha aquatica L. Journal of Essential Oil Bearing Plants 9(3): 283-286.
Phillipson JD, 2007. Phytochemistry and pharmacognosy. Phytochemistry 68: 2960-2972.
Safari F, Seyyed Alangi SZ and Alami H, 2017. Investigation of quantitative and qualitative parameters of dry Mentha aquatica L. by liquid and microwave substrate methods. Journal of Food Science and Technology 9(3): 129-141.
Senator F, Dalessio A, Formisano C and Ozcan M, 2005. Chemical Composition and Antibacterial Activity of the Essential Oil of a 1,8-Cineole Chemotype of Mentha aquatica L. Growing Wild in Turkey. Journal of Essential Oil Bearing Plants 8(2): 148-153.
Sikkema J, De Bont JAM and Poolman B, 1994. Intractions of cyclic hydrocarbons with biological membranes. Journal of Biological Chemistry 269(11): 80022-80028.
Street RA, 2012. Heavy metals in medicinal plant products-An African perspective. South African Journal of Botany 82: 67-74.
Strycharz S and Shetty K, 2002. Peroxidase activity and phenolic content in elite clonal lines of Mentha pulegium in response to polymeric dye R-478 and Agrobacterium rhizogenes. Process Biochemistry 37(8): 805-12.
Thach, L.N., Nhung, T.H., My, V.T.N. and Tran, H.A. (2013). The new rich source of rotundifolone: Mentha aquatica Linn. var. crispa oil from microwave-assisted hydrodistillation. Journal of Essential Oil Research 25(1): 39-43.
Yadegarinia D, Gachkar L, Rezaei MB, Taghizadeh M, Astaneh S and Rosooli I 2006. Biochemical activity of Iranian Mentha piperita L. and Myrtus communis L. essential oils. Phytochemistry 67: 1249-1255.
Zare Dehabadi S, Asrar Z and Mehrabani M, 2010. Biochemical changes in terpenoid compounds of Mentha spicata essential oils in response to excess zinc supply. Iranian Journal of Plant Biology 2(1): 25-34.
Zargari A, 1995. Iranian medicinal plants. Tehran university press. Tehran. Iran 4: 2-38.