Investigation of survival of probiotic Lactobacillus casei and Bifidobacterium lactis encapsulated with calcium alginate-inulin in cold green tea drink

Document Type : Research Paper

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Abstract

Nowadays, food enrichment using probiotic bacteria and prebiotics to increase the population of the bacteria is one of the most important methods in the food industry. The aim of this study was to investigate the viability of probiotic bacteria Lactobacillus casei and Bifidobacterium lactis encapsulated with calcium alginate-inulin in cold green tea drink. In this study, by encapsulating probiotic bacteria Lactobacillus casei and Bifidobacterium lactis with calcium alginate and also adding inulin at different levels (0, 3 and 7% by weight) in green tea drink, a synbiotic product was investigated. During the storage period, probiotic bacteria Lactobacillus casei and Bifidobacterium lactis were counted in MRS-agar medium and physicochemical and sensory properties of the treatments were evaluated. By encapsulating probiotic bacteria and increasing the amount of inulin, the survival of probiotic bacteria was significantly increased compared to the control treatment (P <0.05). The results also showed that there was no significant difference in pH changes in all green tea treatments. Also, the acidity level in green tea treatments containing 3% and 7% inulin at 28 th day was significantly higher than the other amount (P <0.05). Sensory evaluation also showed that there was no significant difference in taste in all green tea treatments. The use of inulin and encapsulation has no effect on organoleptic properties, but increases the survival of probiotic bacteria Lactobacillus casei and Bifidobacterium lactis added to green tea drink. In general, this method can be used to increase the survival of probiotic bacteria during the production and storage of green tea drinks.
Nowadays, food enrichment using probiotic bacteria and prebiotics to increase the population of the bacteria is one of the most important methods in the food industry. The aim of this study was to investigate the viability of probiotic bacteria Lactobacillus casei and Bifidobacterium lactis encapsulated with calcium alginate-inulin in cold green tea drink. In this study, by encapsulating probiotic bacteria Lactobacillus casei and Bifidobacterium lactis with calcium alginate and also adding inulin at different levels (0, 3 and 7% by weight) in green tea drink, a synbiotic product was investigated. During the storage period, probiotic bacteria Lactobacillus casei and Bifidobacterium lactis were counted in MRS-agar medium and physicochemical and sensory properties of the treatments were evaluated. By encapsulating probiotic bacteria and increasing the amount of inulin, the survival of probiotic bacteria was significantly increased compared to the control treatment (P <0.05). The results also showed that there was no significant difference in pH changes in all green tea treatments. Also, the acidity level in green tea treatments containing 3% and 7% inulin at 28 th day was significantly higher than the other amount (P <0.05). Sensory evaluation also showed that there was no significant difference in taste in all green tea treatments. The use of inulin and encapsulation has no effect on organoleptic properties, but increases the survival of probiotic bacteria Lactobacillus casei and Bifidobacterium lactis added to green tea drink. In general, this method can be used to increase the survival of probiotic bacteria during the production and storage of green tea drinks.
Nowadays, food enrichment using probiotic bacteria and prebiotics to increase the population of the bacteria is one of the most important methods in the food industry. The aim of this study was to investigate the viability of probiotic bacteria Lactobacillus casei and Bifidobacterium lactis encapsulated with calcium alginate-inulin in cold green tea drink. In this study, by encapsulating probiotic bacteria Lactobacillus casei and Bifidobacterium lactis with calcium alginate and also adding inulin at different levels (0, 3 and 7% by weight) in green tea drink, a synbiotic product was investigated. During the storage period, probiotic bacteria Lactobacillus casei and Bifidobacterium lactis were counted in MRS-agar medium and physicochemical and sensory properties of the treatments were evaluated. By encapsulating probiotic bacteria and increasing the amount of inulin, the survival of probiotic bacteria was significantly increased compared to the control treatment (P <0.05). The results also showed that there was no significant difference in pH changes in all green tea treatments. Also, the acidity level in green tea treatments containing 3% and 7% inulin at 28 th day was significantly higher than the other amount (P <0.05). Sensory evaluation also showed that there was no significant difference in taste in all green tea treatments. The use of inulin and encapsulation has no effect on organoleptic properties, but increases the survival of probiotic bacteria Lactobacillus casei and Bifidobacterium lactis added to green tea drink. In general, this method can be used to increase the survival of probiotic bacteria during the production and storage of green tea drinks.
Nowadays, food enrichment using probiotic bacteria and prebiotics to increase the population of the bacteria is one of the most important methods in the food industry. The aim of this study was to investigate the viability of probiotic bacteria Lactobacillus casei and Bifidobacterium lactis encapsulated with calcium alginate-inulin in cold green tea drink. In this study, by encapsulating probiotic bacteria Lactobacillus casei and Bifidobacterium lactis with calcium alginate and also adding inulin at different levels (0, 3 and 7% by weight) in green tea drink, a synbiotic product was investigated. During the storage period, probiotic bacteria Lactobacillus casei and Bifidobacterium lactis were counted in MRS-agar medium and physicochemical and sensory properties of the treatments were evaluated. By encapsulating probiotic bacteria and increasing the amount of inulin, the survival of probiotic bacteria was significantly increased compared to the control treatment (P <0.05). The results also showed that there was no significant difference in pH changes in all green tea treatments. Also, the acidity level in green tea treatments containing 3% and 7% inulin at 28 th day was significantly higher than the other amount (P <0.05). Sensory evaluation also showed that there was no significant difference in taste in all green tea treatments. The use of inulin and encapsulation has no effect on organoleptic properties, but increases the survival of probiotic bacteria Lactobacillus casei and Bifidobacterium lactis added to green tea drink. In general, this method can be used to increase the survival of probiotic bacteria during the production and storage of green tea drinks.

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References

AFRC, R F. 1989. Probiotics in man and animals. Journal of Applied Microbiology 66(5): 365-378.
Cardarelli HR, Buriti FCA, Castro IA, and Saad SMI. 2008. Inulin and oligofructose improve sensory quality and increase the probiotic viable count in potentially synbiotic petit-suisse cheese. LWT-Food Science and Technology 41(6): 1037–1046.
Chaikaew S, Baipong, S, Sone T, Kanpiengjai A, Chui-Chai N, Asano K and Khanongnuch C. 2017. Diversity of lactic acid bacteria from Miang, a traditional fermented tea leaf in northern Thailand and their tannin-tolerant ability in tea extract. Journal of Microbiology 55(9): 720-729.
Chávarri M, Marañón I, Ares R, Ibáñez F C, Marzo F and del Carmen Villarán M. 2010. Microencapsulation of a probiotic and prebiotic in alginate-chitosan capsules improves survival in simulated gastro-intestinal conditions. International journal of food microbiology 142(1-2): 185-189.
De Lacey AL, Pérez-Santín E, López-Caballero ME\ and Montero P. 2014. Survival and metabolic activity of probiotic bacteria in green tea. LWT-Food Science and Technology 55(1): 314-322.
Doleyres Y, Lacroix C. 2005. Technologies with free and immobilized cells for probiotic bifidobacteria production and protection. International Dairy Journal 15: 973–988.
Gandomi H, Abbaszadeh S, Misaghi A, Bokaie S, and Noori N. 2016. Effect of chitosan-alginate encapsulation with inulin on survival of Lactobacillus rhamnosus GG during apple juice storage and under simulated gastrointestinal conditions. LWT-Food Science and Technology 69: 365-371.
González-Forte L, Bruno E, and Martino M. 2014. Application of coating on dog biscuits for extended survival of probiotic bacteria. Animal Feed Science and Technology 195: 76-84.
IDF 1991. Yogurt: determination of titratable acidity- potentiometric method. International Dairy Federation Standard 150. Brussels – Belgium.
Hashemi FS, Gharibzahedi SMT, and Hamishehkar H. 2015. The effect of high methoxyl pectin and gellan including psyllium gel on Doogh stability. RSC Advances 5(53): 42346–42353.
Homayouni A, Azizi A, Ehsani MR, Yarmand MS, and Razavi SH.2008. Effect of microencapsulation and resistant starch on the probiotic survival and sensory properties of synbiotic ice cream. Food chemistry 111(1):50-5.
Kailasapathy K. 2006. Survival of free and encapsulated probiotic bacteria and their effect on the sensory properties of yoghurt. LWT-Food Science and Technology 39(10):1221-1227.
Kailasapathy K, and Sultana k. 2003. Survival and b-D-galactosidase activity of encapsulated and free Lactobacillus acidophilus and Bifidobacterium lactis in ice cream. Australian Journal of Dairy Technology 58(3): 223–227.
Krasaekoopt W, Bhandari B, and Deeth H. 2004. The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria. International dairy journal 14(8): 737-743.
Krasaekoopt W, Bhandari B, and Deeth H. 2006. Survival of probiotics encapsulated in chitosan-coated alginate beads in yoghurt from UHT- and conventionally treated milk during storage. LWT 39(6): 177-183.
Krasaekoopt W, Bhandari B, and Deeth H. 2003. Evaluation of encapsulation techniques of probiotics for yoghurt. International Dairy Journal 13(1): 3-13.
Mazloumi SM, Shekarforoush SS, Ebrahimnejad H, and Sajedianfard J. 2011. Effect of adding inulin on microbial and physico-chemical properties of low fat probiotic yogurt. Iranian Journal of Veterinary Research 12(2):93-98.
Mirzaei H, Pourjafar H, and Homayouni A. 2012. Effect of calcium alginate and resistant starch microencapsulation on the survival rate of Lactobacillus acidophilus La5 and sensory properties in Iranian white brined cheese. Food Chemistry 132(4): 1966-1970.
Molan AL, Flanagan J, Wei W and Moughan PJ. 2009. Selenium-containing green tea has higher antistidant and prebiotic activities than regular green tea. Food Chemistry 829:114–835.
Özcan M M, Ünver A, Uçar T, and Arslan D. 2008. Mineral content of some herbs and herbal teas by infusion and decoction. Food Chemistry 106(3): 1120-1127.
Shah NP and Ravula R R. 2000. Microencapsulation of probiotic bacteria and their survival in frozen fermented dairy desserts. Australian Journal of Dairy Technology 55:139–144.
Singh G, Kapoor I PS and Singh, P. 2011. Effect of volatile oil and oleoresin of anise on the shelf life of yogurt. Journal of Food Processing and Preservation 35(6): 778-783.
Tharmaraj N, and Shah N P. 2004. Survival of Lactobacillus acidophilus, Lactobacillus paracasei subsp. paracasei, Lactobacillus rhamnosus, Bifidobacterium animalis and Propionibacterium in cheese-based dips and the suitability of dips as effective carriers of probiotic bacteria. International Dairy Journal 14(12): 1055-1066.
Vodnar D and Socaciu C. 2012. Green tea increases the survival yield of Bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions. Chemistry Central Journal 6-61.
Zanjani M A K, Tarzi BG, Sharifan A, and Mohammadi N. 2014. Microencapsulation of probiotics by calcium alginate-gelatinized starch with chitosan coating and evaluation of survival in simulated human gastro-intestinal condition. Iranian journal of pharmaceutical research IJPR 13(3): 843.
Zubillaga M, Weill R, Postaire E, Goldman C, Caro R, and Boccio J. 2001. Effect of probiotics and functional foods and their use in different diseases. Nutrition Research 21(3): 569-579.
Food Research Journal
Volume 32, Issue 1
December 2021
Pages 137-149

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