The effects of edible coating of basil seed gum and caraway essential oil on microbial and chemical characteristics of Silver carp fillets

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Food Science and Technology, Birjand Branch, Islamic Azad University, Birjand, Iran

2 M.Sc. Graduate of Food Science and Technology, Birjand Branch, Islamic Azad University, Birjand, Iran

10.22034/fr.2024.58297.1900

Abstract

Introduction : Consumption of seafood has increased during recent years as consumers have become more aware of its nutritional benefits and of the health concerns associated with other meat products such as chicken and beef . Fish is a more perishable product than other muscle foods and its freshness degrades after death due to various biochemical reactions (e.g. changes in protein and lipid content, and the formation of biogenic amines and hypoxanthine ) and microbiological spoilage ( Matak et al., 2015 ). Spoilage of fish results from changes brought about by biological reactions such as oxidation of lipids , the activities of the fish muscular enzymes and the metabolic activities of microorganisms ( Bagheri et al., 2016 ). Nowadays , to maintain the safety and quality of foods , long-term storage of the product and prevent the spread of foodborne diseases , the use of anti-microbial substances in food products has developed a lot ( Appendini and Hotchkiss, 2002) . Using anti-oxidant and anti-microbial preservatives in meat and meat products is very common to prevent the lipid oxidation and spoilage and increased the shelf life and quality of products ( Rashidaie et al., 2019 ) . The use of chemical preservatives in food is harmful to the health of consumers and leads to the occurrence of dangerous diseases such as types of cancers , on the other hand with the increase in environmental warnings , much attention has been paid to the use of biodegradable and environmentally friendly packaging films under the title of edible films and coating , thus food industry manufacturers and researchers are looking for the use of natural preservatives to increase the shelf life and maintain the quality of foods( Joerger, 2007 ) . Natural anti-microbials from different sources are used to preserve food from spoilage and pathogenic microorganisms . Many plants have been considered as the source of natural anti-oxidants due to their effective compounds such as polyphenolic compounds , flavonoids , tannins and phenolic acids . In addition to the anti-oxidant properties, these compounds also have anti-microbial, anti-cancer, and anti-mutagenic properties ( Rashidaie et al.,2019 ) . Hypophthalmichthys molitrix is widely used in Freshwater fish breeding systems around the world, due to its fast growth, stress resistance and high nutritional value ( Khorrami et al., 2013 ) . In Iran , in recent years, the breeding rate of this species has been on the rise . Microbial contamination and physicochemical reactions have an effective role in reducing the edible quality of various types of fish and edible coating based on natural compounds can increase the shelf life of the products ( Arshad and Batool, 2017 ) . This research was conducted to investigate the effect of the edible coating of basil seed gum and various concentrations of caraway essential oil on the microbial and chemical characteristics of Hypophthalmichthys molitrix fillet during storage in the refrigerator temperature . The benefits of basil seed gum compared to other polysaccharides are its hydrophilic, bio compatible and cost effective properties ( Khazaeia et al.,2014 ). Basil has a high amount of phenolic compounds and as a result it's anti-oxidative properties is high . It also has antimicrobial effects ( Phuong et al.,2010 ) . Cuminaldehyde, γ-Terpinene and p-cymene are among the effective compounds in caraway essential oil which have antifungal, antibacterial and antioxidant effects (AL-Jabri and Hossain,2014), moreover It has anti-allergic effects, reduces blood cholesterol and strengthens the body’s immune system ( Brody et al., 2008 ).
Material and methods : In this study the control sample (fish fillet without coating) and treatments coated with 5% basil seed gum and 0.25, 0.5 and 1% caraway essential oil where evaluated . The basil seeds were carefully cleaned removing dusts, stones and chaffs. The gum extraction conditions were as follows : 70 °C temperature, pH =7, 20-minute soaking time and a water to seed ratio of 65:1. The Caraway essential oil was obtained by hydro-distillation of the seeds during 3 hours using a Clevenger-type apparatus. During 15 days of the storage in the refrigerator and at intervals of 3days microbial tests, including the psychrotrophic and total bacterial count, and physicochemical tests, including measuring the amount of pH , Thiobarbituric acid (TBA ) and total volatile basic nitrogen (TVB-N ) were performed . The thiobarbituric acid reactive substances test is a method to investigate secondary oxidative products especially in polyunsaturated fatty acids such as malonaldehydes . The TVB-N value is one of the most widely used indicators of seafood deterioration . It is a general term which includes the calculation of three methyl amine ( produced by spoilage bacteria ), dimethyl amine ( produced by autolytic enzymes during chilled preservation ) , ammonia (produced by the deamination of amino acids and nucleotide catabolizes ) as well as other volatile basic nitrogenous compounds correlated with seafood spoilage ( Erikson et al., 2011) . All the tests were carried out in triplicates and the results were expressed as mean values and standard deviation. The analysis of variance was applied to the data and the means were compared by Duncan’s test using SPSS statistical software .
Results and discussion : Increasing the concentration of caraway essential oil in the edible coating the fish fillets during 15 days of the storage in the refrigerator had the positive effect on the reduction of psychrotrophic bacteria and total microbial count, so that by increasing the concentration of caraway essential oil the microbial load decreased and this difference was statistically significant (P<0.05 ). In addition, the amount of pH , Thiobarbituric acid and total volatile basic nitrogen in the coated samples were significantly more favorable than the control sample. During the storage of treatments in the refrigerator, the pH level increased . There was no significant difference between the pH of the control sample and the other treatments until the Sixth Day . On days 9 and 12, the pH of control sample was significantly higher than the treatments containing 0.5% and 1% essential oil . On the 15th day, the minimum and maximum pH values were evaluated in the treatment containing 1% essential oil and the control sample, respectively, while the difference in the investigated factor was significant in all the samples ( P<0.05 ) . The amount of TBA increased in all treatments during the storage period in the refrigerator. In the days of 3-15 , the TBA value in the control sample was significantly more than other treatments . On days 6 to 15, in treatments with more caraway essential oil , the TBA value was significantly less than in other treatments . The highest and lowest amount of TBA was determined in the control sample and treatment containing 1 % caraway essential oil, respectively . TVB-N index mainly represents the quality of fish and the expression presence of nitrogen compounds from destruction molecules such as proteins and nucleic acids by proteolytic bacteria . In all samples , the amount of TVB-N increased during the storage period in the refrigerator. On days 0 and 3, there was no statistically significant difference in the amount of total volatile basic nitrogen in the samples . From the Sixth Day, the amount of TVB-N in the control sample was significantly higher than the coated samples . The increase in the amount of TVB-N during the storage period can be related to the activity of bacteria and internal enzymes that accelerate the spoilage process and the accumulation of trimethylamine , dimethylamine , ammonia and other nitrogenous bases . The use of essential oil in the coating of fish fillets was effective in reducing the total amount of volatile basic nitrogen , compared to the control sample .
Conclusion : The results of this research showed that the use of an edible coating of basil seed gum and caraway essential oil reduces the intensity of bacterial activity in fish fillet samples and increases its shelf life in the refrigerator . The speed of quality reduction in treatments was significantly lower than in the control sample . The use of an edible coating of basil seed gum and 1% caraway essential oil improved the microbial and chemical characteristics and increased the shell life of fish fillets for 12 days in the refrigerator . Considering the disadvantages of chemical preservatives for consumers and to increase the shelf life of fish, it is recommended to use edible coating of basil seed gum and caraway essential oil .

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Main Subjects


اجاق م، رضایی م، رضوی ه و حسینی م ه،1391.  اثر پوشش­های آنتی میکروبی در افزایش ماندگاری ماهی قزل­آلای رنگین­کمان. مجله علوم و صنایع غذایی ایران، ۹ (۳۴)، 23-13          URL: http://fsct.modares.ac.ir/article-7-6776-fa.html
استاندارد ملی ایران، 1386. روغن­ها و چربی­های گیـاهی و حیوانی- اندازه­گیری عدد2- تیوباربیتوریک اسید به روش مستقیم. سازمان ملی استاندارد، شماره 10494
استاندارد ملی ایران،1397. میکروبیولوژی زنجیره مواد غذایی- آماده­سازی آزمایه­ها. سوسپانسیون اولیه و رقت­های اعشاری برای آزمون میکروبی- قسمت3: مقررات ویژه برای آماده­سازی ماهی و فرآورده­های آن-تجدید نظر اول، سازمان ملی استاندارد، شماره 3-8923.
استاندارد ملی ایران،1393. میکروبیولوژی زنجیره مواد غذایی- روش جامع برای شمارش میکروارگانیسم­ها-قسمت1- شمارش کلنی در30 درجه سلسیوس با استفاده از روش کشت آمیخته، سازمان ملی استاندارد، شماره 1-5272
استاندارد ملی ایران، 1382، میکروبیولوژی مواد غذایی و خوراک دام- شمارش میکروارگانیسم­های سرماگرا- روش آزمون، سازمان ملی استاندارد ، شماره 2629
پورکارگر ه و رفعتی ع،1399. اثر پوشش خوراکی کیتوزان حاوی اسانس نعناع فلفلی (Mentha piperita L.) بر ماندگاری فیله ماهی قزل­آلای رنگین‌کمان ­(Oncorhynchus mykiss)­ طی نگهداری در دمای یخچال. مجله بوم­شناسی آبزیان،۱۰ (۱) ،۶۶-53                                      URL: http://jae.hormozgan.ac.ir/article-1-871-fa.html
تقی­زاده اندواری ق و رضایی م، 1391. اثر پوشش ژلاتینی بر ویژگی­های شیمیایی، میکروبی و حسی فیله ماهی قزل­آلای رنگین کمان (Oncorhynchus mykiss)­دردمای یخچال. مجله علوم و صنایع غذایی ایران، ۹ (۳۷)، ۶۷-۷۶
خرمی م، حسینی­پرور س ه و معتمدزادگان ع، 1392. بررسی اثر صمغ دانه ریحان بر روی پایداری، رفتار رئولوژیکی و اندازه ذرات امولسیون­های ایزوله پروتئین آب پنیری. نشریه فرآوری و نگهداری مواد غذایی، 5(1)، 114-91 .
ربیعی ث، حسینی ه و رضائی م، 1393. اثر بازدارندگی اسانس زیره سیاه بر رشد باکتری لیستریا مونوسیتوژنز در محیط­‌های مدل شبیه‎سازی‌شده و گوشت ماهی سفید دریای خزر­­(Rutilus frisii kutum) . نشریه پژوهش­های علوم و صنایع غذایی ایران، 2(10)، 128-122                     doi: 10.22067/ifstrj.v10i2.37816
رفیعی­پور ا، نژاد­سجادی ح، شهدادی ف، مهدوی­نیا ع، دزیانی م وعزتی ر، 1398. تاثیر اسانس زیره سیاه و آویشن بر ویژگی­های کیفی و ماندگاری ماهی منجمد و تازه. مجله علوم و صنایع غذایی ایران،۱۶ (95)، 63-74  
زمانی ع و غفاری آ، 1398. بررسی کیفیت شیمیایی وباکتریایی سوریمی تهیه­شده از ماهی کیلکای معمولی (Clupeonella cultriventris) حاوی عصاره پونه (Mentha pulegium) طی نگهداری در یخچال (1-4درجه سانتیگراد)، مجله علمی شیلات ایران، 28(4)، 35-23
سحری م ع و صالحی ح،1400. بررسی اثر استفاده از پوشش پروتئینی ژلاتین به‌همراه عصاره گل میخک برای افزایش کیفیت نگهداری فیله ماهی قزل‌آلای سرد شده در دمای۴ درجه سلسیوس. مجله علوم و صنایع غذایی ایران. ۱۸ (۱۱۰) ،۱۱-۲۵ 
/article-7-16993-fa.htm URL: http://fsct.modares.ac.ir
سیاری ز، ربانی م، فرهمندفر ر، اسماعیل زاده کناری ر و موسوی ندوشن ر،1400. اثر اسانس دانه زیر سیاه   (Bunium persicum) انکپسوله شده با صمغ بذر ریحان (Ocimum basilicum L.) بر تغییرات شیمیایی، میکروبی و حسی فیله ماهی قزل آلا رنگین کمان نگهداری شده در دمای یخچال.  مجله علوم و صنایع غذایی ایران، ۱۸ (۱۱۸) ،۱۲۹-۱۴۰ . doi: 10.52547/fsct.18.118.129     
شباهنگ ز، بهرامی س و محمدی ب، 1398. تهیه فیلم نانوکامپوزیت کیتوزان- نانوذرّات اکسید روی-اسانس آویشن و بررسی ویژگی‌های مکانیکی، ساختاری و ضدمیکروبی آن. فصلنامه علمی علوم و فنون بسته­بندی، 10(40)، 58-73.
شعبانپور ب، اصغری م، , حیدری س، بایی ه،  قربانی, آ و جافر ع،1394. مقایسه تغییرات کیفی فیله کپورماهیان پرورشی در محیط استخر و آزمایشگاه و ماهی کپور دریایی طی نگهداری در یخچال  پژوهش‌های جانوری. مجله زیست شناسی ایران، 28(4)، 480-466
علی­محمدزاده م ر، علیدوست م ع و خندقی ج،­1399. مطالعه اثر ضدمیکروبی عصاره الکلی و اسانس زیره سیاه Bunium persicum boiss ) بر برخی از باکتری­ها و کپک­ها در پنیر لاکتیکی. مجله میکروب­شناسی مواد غذایی، 7(4)، 33-46
قصیری ش، هدایتی­فرد م و معتمدزادگان ع، 1393. تولید کتلت ماهی از کپور سرگنده (Aristichthys nobilis) و بررسی ویژگی‌های شیمیایی و میکروبی محصول منتخب طی دوره نگه‌داری در شرایط انجماد، مجله علمی بهداشت مواد غذایی، 4(13)، 33-45       
گرجیان ه و رفتنی امیری ز، 1398. تأثیر موسیلاژ دانه‌ شاهی بر پایداری و خواص رئولوژیکی دوغ بدون چربی. پژوهش های صنایع غذایی، 29(3) ، 145-156    
محمدزاده ب و رضایی م،1392. اثر پلی­فنل­های چای سبز بر تغییرات میکروبی و شیمیایی ماهی قزل‌آلای ‌رنگین‌ کمان­(Oncorhynchus mykiss)  به هنگام نگهداری در یخ. مجله علوم و صنایع غذایی ایران، ۱۰ (۳۸) ،۹-1   
محمدی ب،1399. سنتز نانو کامپوزیت کیتوزان- پلی­آنیلین و بررسی ویژگی­های ساختاری و زیستی و ضد باکتریایی آن. فصلنامه علمی علوم و فنون بسته بندی، 11(42)،80-92    
میرعرب­رضی س، معتمدزادگان ع، شهیدی س ا و رشیدی­نژاد ع، 1398. بهبود ویژگی­های ژلی آلبومین سفیده تخم مرغ با افزودن صمغ دانه ریحان. نشریه پژوهش­های صنایع غذایی، 29(4)، 12-1.
ندائی م، نوری قنبلانی ق، رفیعی دستجردی ه و عباداللهی ع، 1399.  اثرهای کشندگی و زیرکشندگی اسانس‌های درمنه خراسانی­(Artemisia khorassanica) ، زیره‌ سیاه (Carum carvi) و فلفل سیاه (Piper nigrum) روی بید سیب‌زمینی(Phthorimaea operculella)  ، مجله علمی تحقیقات آفات گیاهی،10(4)،41-57        doi: 10.22124/iprj.2021.4631
AL-Jabri NN and Hossain MA, 2014. Comparative chemical composition and antimicrobial activity study of essential oils from two imported lemon fruits samples against pathogenic bacteria. Beni-Suef University Journal of Basic and Applied Sciences 3(4): 247-253.      doi: 10.1016/j.bjbas.2014.10.011
Appendini P and Hotchkiss JH, 2002. Review of antimicrobial food packaging. Innovative Food Science and Emerging Technologies. 3(2): 113-126.‏        https://doi.org/10.1016/S1466-8564(02)00012-7
Arshad MS and Batool SA, ­­2017. ­Natural antimicrobials, their sources and food safety. Food Additives 87(1).     doi: 10.5772/intechopen.70197
Bagheri R, Izadi Amoli R­, Tabari Shahndash N and Shahosseini S R, 2016. Comparing the effect of encapsulated and unencapsulated fennel extraction the shelf life of minced common kilka (Clupeonella cultriventris caspia) and Pseudomonas aeruginosa inoculated in the mince. Food Science and Nutrition 4(2): 216–222.         doi: 10.1002/fsn3.275
Berizi E, Hosseinzadeh S, Shekarforoush SSH and Barbieri G, 2018. Microbial, chemical, textural and sensory properties of coated rainbow trout by chitosan combined with pomegranate peel extract during frozen storage. International Journal of Biological Macromolecules 106: 1004-1013.  doi: 10.1016/j.ijbiomac.2017.08.099
Bourgou S, Pichette A, Marzouk B and Legault J, 2010. Bioactivities of black cumin essential oil and its main terpenes from Tunisia. South African Journal of Botany 76(2): 210-216.  https://doi.org/10.1016/j.sajb.2009.10.009
Brody AL, Bugusu B, Han JH, Sand CK and McHugh T H, 2008. Innovative food packaging solutions. Scientific Status Summary. Journal of Food Science 73(8): 107-116    https:// doi: 10.1111/j.1750-3841.2008. 00933.x
Chamanara V, Shabanpour B and Khomeiri M, 2012. An investigation on characteristics of rainbow trout coated using chitosan assisted with thyme essential oil. International Journal of Biological Macromolecules 50(3): 540-544.     https://doi.org/10.1016/j.ijbiomac.2012.01.016
Chytiri S, Chouliara I, Savvaidis IN and Kontominas MG, 2004. Microbiological, chemical and sensory assessment of iced whole and filleted aquaculture rainbow trout. Food Microbiology 21:157-165.      doi:10.1016/S0740-0020(03)00059-5
Erikson U, Misimi E and Gallart-Jornet L, 2011. Superchilling of rested Atlantic salmon: different chilling strategies and effects on fish and fillet quality. Food Chemistry 127(4): 1427–1437. doi:10.1016/j.foodchem.2011.01.036
Goʹmez-Estaca J, Lopeʹz de lacey A, Goʹmez-Guilleʹn MC, Lopeʹz-Caballero ME and Montero P, 2009. Antimicrobial activity of composite edible films based on fish gelatin and chitosan incorporated with clove essential oil. Journal of Aquatic Food Product Technology 18(1-2):46-52.   doi: 10.1080/10498850802581252
Ibrahim SM and El-Sherif SA, 2008. Effect of some extracts on quality aspects of frozen tilapia (Oreochromis niloticus L.) fillets. Global Vetrinaria 2(2): 62-66.
Joerger RD, 2007. ­Antimicrobial films for food applications: a quantitative analysis of their effectiveness. Packaging Technology and Science: An International Journal 20(4): 231-273. ‏https://doi.org/10.1002/pts.774
Jouki M, Yazdi FT, Mortazavi SA, Koocheki A and Khazaei N, 2014. Effect of quince seed mucilage edible films incorporated with oregano or thyme essential oil on shelf life extension of refrigerated rainbow trout fillets. International Journal of Food Microbiology 174: 88-97.  doi: 10.1016/j.ijfoodmicro.2014.01.001
Khazaeia N, Esmaiili M, Emam Djomeh Z, Ghasemlou M and Jouki, M, 2013. Characterization of new biodegradable edible film made from basil seed (Ocimum basilicum L.) gum. Carbohydrate Polymers 102: 199– 206.     doi: 10.1016/j.carbpol.2013.10.062
Khorrami M, Hosseini Parvar SH and Motamed Zadegan A, 2013. Effect of basil gum on the stability, rheological behavior and particle size of whey protein isolate emulsions. Journal of Food Processing and Maintenance 5 (2): 91-114
Kyrana VR, Lougovois VP and Valsamis DS,1997. Assessment of shelf life of mari cultured gilthead sea bream (Sparus aurata) stored in ice. International Journal Food Science and Technology 32(4):339 – 347       doi: 10.1046/j.1365-2621.1997.00408.x
Lee C H and Chin K B, 2020. Physical properties and structural changes of myofibrillar protein gels prepared with basil seed gum at different salt levels and application to sausages. Foods 9(6): 702 https://doi.org/10.3390/foods9060702
Lin C, and Lin C,2005. Enhancement of the storage quality of frozen bonito fillets by glazing with tea extracts. Food Control 16: 169–175.        doi:10.1016/j.foodcont.2004.01.007
Matak K E, Tahergorabi R, and Jaczynski J,2015. A review: Protein isolates recovered by isoelectric solubilization /precipitation processing from muscle food by‐products as a component of nutraceutical foods. Food Research International 77(4): 697–703.       doi.org/10.1016/j.foodres.2015.05.048
Mexis SF, Chouliara E and Kontominas MG, 2009. Combined effect of an oxygen absorber and oregano essential oil on shelf-life extension of rainbow trout fillets stored at 4°C. Food Microbiology 26: 598-605.     doi: 10.1016/j.fm.2009.04.002
Pavli F, Tassou C, Nychas G E and Chorianopoulos N, 2018. Probiotic Incorporation in Edible Films and Coatings: Bioactive Solution for Functional Foods, International Journal of Molecular Sciences 19(1): 150.     doi: 10.3390/ijms19010150
Phuong MN, Eileen M K and Emily DN, 2010. Potassium rate alters the antioxidant capacity and phenolic concentration of basil (Ocimum basilicum L.) Leaves. Journal of Food Chemistry 123(4):1235-1241.       doi: 10.1016/j.foodchem.2010.05.092
Ramos M, Jiménez A, Peltzer M and Garrigós M C, 2012. Characterization and antimicrobial activity studies of polypropylene films with carvacrol and thymol for active packaging. Journal of Food Engineering 109(3): 513-519.‏       https://doi.org/10.1016/j.jfoodeng.2011.10.031
Rashidaie Abandansarie SS, Ariaii, P and Charmchian Langerodi M, 2019. Effects of encapsulated rosemary extract on oxidative and microbiological stability of beef meat during refrigerated storage. Food Science and Nutrition. 7 (12): 3969-3978.    doi: 10.1002/fsn3.1258
Song Y, Liu L, Shen H, You J and Luo Y, 2011. Effect of sodium alginate-based edible coating containing different anti-oxidants on quality and shelf life of refrigerated bream (Megalobrama amblycephala). Food Control 22(3): 608-615.      doi: 10.1016/j.foodcont.2010.10.012
Sonmezdag AS, Amanpour A, Kelebek H and Selli S, 2018. The most aroma-active compounds in shade-dried aerial parts of basil obtained from Iran and Turkey. Industrial Crops and Products 124: 692-698. ‏https://doi.org/10.1016/j.indcrop.2018.08.053
Vasconez MB, Flores SK, Campson CA, Alvarado J and Gerschenson LN, 2009. Antimicrobial activity and physical properties of chitosan-tapioca starch based edible films and coatings. Food Research International 42(7):762-769            doi: 10.1016/j.foodres.2009.02.026