Effect of melatonin postharvest application on nutritional quality and shelf life of fresh-cut mushrooms (Agaricus bisporus)

Document Type : Research Paper

Authors

1 Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Iran.

2 Dep.of Horticultural Sciences, Faculty of Agriculture, University of Tabriz. Tabriz, Iran.

3 Department of Horticultural Science, Imam Khomeini International University, Qazvin

4 Seed and Plant Certification and Registration Institute, Karaj

Abstract

Introduction: Button mushroom (Agaricus bisporus) is approved in the global food market, with regards for 30% of total mushroom production in the world, and high content of bioactive constituents including vitamins, minerals, essential amino acids as well as valuable source of polysaccharide (Meng et al., 2012). The market for sliced fresh mushrooms is increasing rapidly due to consumer demand for prepared ready-to-use vegetables and convenience foods. Button mushroom was considered as a highly perishable food due to high respiration rate, and actually no natural physical barrier on its surface to protect it, which leading to texture softening, moisture loss and browning during postharvest (Brennan et al., 2000). For sliced fresh mushrooms, the shelf life is even shorter because of the effects of the washing and cutting processes. Melatonin (N-acetly-5-methoxytryptamine) is an endogenously beneficial indole amine synthesized by all cellular organisms, which can reduce the physiological detrimental effect of abiotic stresses in plants (Zhang et al., 2018). With this regard, melatonin can play prominent roles, as a highly efficient antioxidant, scavenging molecule, and as a signaling molecule in regulating or adjusting ROS scavenging enzyme activities (Zhang et al., 2018). Recently, numerous studies reported that exogenous melatonin treatment besides storage at low temperature has been improved postharvest preservation of several fruit and vegetables (Hu et al., 2018). Since there is no information about the response of fresh-cut button mushrooms to oxidative stress following exogenous melatonin treatment during postharvest cold storage, we evaluated the effects of exogenous melatonin on qualitative attributes of fresh-cut button mushroom with regard to cap browning, weight loss, membrane permeability indicators and phenol metabolism during postharvest low temperature storage.
Material and methods: Button mushrooms were selected on the basis of the uniformity of maturity and size and any mechanical wounded, and were then hand sliced using a sharp knife (4 mm wide). The sliced fresh mushrooms were immersed in 0, 1, 10, 100 and 1000 µmol/L of melatonin solution for 5 minutes at 20 °C, then removed from melatonin solution and air dried at room temperature for 1 hr. After that, sliced mushrooms of each replication were packed in 500g pet plastic mushroom box (40 sliced mushrooms per box) and stored in darkness at 4 °C with 90-95% relative humidity. During storage period (5, 10 and 15 days) sliced mushroom caps per replication of each treatment were used for estimating of traits. The button mushroom weight loss was determined during storage period. To calculate the browning index (BI), photographs of edible mushroom samples were taken on sampling days and L, a and b were calculated in Photoshop software environment. Weight loss was measured according to the method of Nasiri et al (2017). A spectrophotometric procedure described by Terada et al (1978) used for the determination of ascorbic acid content. Total phenolics was measured with using the Folin–Ciocalteu reagent by Singleton and Rossi (1965) method. The texture of the mushroom cap was used to measure electrolyte leakage content and determined essentially as described by Meng et al (2012). Malondialdehyde accumulation was measured with using the thiobarbitoric acid reagent according to the method of Hodges et al (1999). DPPH radical was used to measure of scavenging activity according to the method of Dokhanieh and Aghdam (2016). The extraction and activity of PAL were determined using method described by Nguyen et al (2003). PPO was extracted with acetone according to Nguyen et al. (2003). One-way analysis of variance and Duncan’s multiple range test at P<0.05 were used for multiple mean comparisons with SAS software.
Results and discussion: Our results showed that weight loss and cap browning index of fresh cut mushrooms following exogenous melatonin treatment (10 µM), was significantly declined to lower amount than the control during cold storage at 4 °C for 15 days. According to our results, exogenous melatonin treatment significantly slowed process of senescence in strawberry fruit as exhibited by reduced weight loss and cap browning softening of during storage (Liu et al., 2018). At the end of storage, application of endogenous melatonin at 100 µmol/L led to an increase in total phenol and ascorbic acid in fresh cut button mushrooms. Similar enhancements in total phenol and ascorbic acid along with quality retention have also been reported in exogenous melatonin treated of peach and strawberry fruit during storage time (GAO et al., 2016; Aghdam & Fard, 2017). In this study, exogenous melatonin treatment obviously declined the increasing rate of electrolyte leakage rate as well as MDA contents compared to the control, demonstrating that the effect of melatonin in reduction of membrane oxidative damage might lead to slow down browning and senescence in fresh cut mushrooms. In agreement with this suggestion, suppression of oxidative stress due to retarded senescence and quality deterioration has been described in melatonin treated cassava root and strawberry and pomegranate fruit (Ma et al., 2106; Liu et al., 2018). DPPH scavenging capacity of melatonin treated fresh cut mushrooms steadily remained higher than the control during cold storage. Higher phenylpropanoid pathway activity in response to exogenous melatonin treatment during storage, might lead to accumulation of phenolic compounds which in result provided free radical scavenging capacity up-regulation (Zheng et al., 2019). As shown in this study, higher PAL and lower PPO enzymes activity in fresh cut mushrooms followed by exogenous melatonin treatment, may be associated with molecular signaling of H2O2 pool due to enhanced NADPH oxidase enzyme activity, result in triggering phenylpropanoid pathway might lead to higher total phenol content and higher total antioxidant scavenging activity, as well membrane lipids protection from peroxidation which indicated by enhanced PAL/PPO enzymes activity accompanying with lowering cap browning (Rastegar et al., 2020).
Conclusion: In summary, results of this study revealed the functional effects of postharvest exogenous melatonin application at 10 µM in preserving fresh cut mushrooms quality by retarding senescence process in A.bisporus as demonstrated by attenuating weight loss and cap browning during cold storage at 4 °C for 15 days. Bioactive constituents such as ascorbic acid and total phenolics notably enhanced in response to exogenous melatonin, which is accompanied with higher PAL and lower PPO activities in melatonin treated fresh cut mushrooms during storage time. Melatonin application maintained cell membrane stability as exhibited by lowering electrolyte leakage, MDA values as well as enhancing total antioxidant activity. In general, our result showed exogenous melatonin treatment can be used as a safe and beneficial method to maintain button mushrooms quality, reduce the cap browning and prolong postharvest life the mushrooms.

Highlights

Aghdam MS and Fard JR, 2017. Melatonin treatment attenuates postharvest decay and maintains nutritional quality of strawberry fruits (Fragaria × anannasa cv. Selva) by enhancing GABA shunt activity. Food Chemistry 221: 1650–1657.

Aghdam MS, Luo Z, Jannatizadeh A and Farmani B, 2019. Exogenous adenosine triphosphate application retards cap browning in Agaricus bisporus during low temperature storage. Food chemistry 293: 285-290.

Arnao MB and Hernández-Ruiz J, 2017. Melatonin and its relationship to plant hormones. Annal Botany 121: 195–207.

Baeza R, 2007. Comparison of thechnologies to control the physiologica, biochemical and nutritional changes of fresh cut fruit.  Food Science Graduate Program. College of Agriculture. Pp: 109.

Brennan MH, Le Post G and Gormley TR, 2000. Postharvest treatment with citric acid and hydrogen peroxide to extend the shelf life of fresh sliced mushrooms. Lebensm-Wiss. U Technology 33: 285–289.

Cao S, Shao J, Shi L, Xu L, Shen Z, Chen W and Yang Z, 2018. Melatonin increases chilling tolerance in postharvest peach fruit by alleviating oxidative damage. Scientific Reports 8: 806.

Cantos E, Tudela JA, Gil MI and Espin JC, 2002. Phenol compounds and related enzymes are not rate-limiting in browning development of fresh-cut potatoes. Journal of Agricultural and Food Chemistry 50: 3015–3023.

Dokhanieh AY and Aghdam MS, 2016. Postharvest browning alleviation of Agaricus bisporus using salicylic acid treatment. Scientia Horticulturae 207: 146–151.

Fan J, Hu Z, Xie Y, Chan Z, Chen K, Amombo E, Chen L and Fu J, 2015. Alleviation of cold damage to photosystem II and metabolisms by melatonin in Bermudagrass. Frontiers in Plant Science 6: 925.

Gao H, Zhang ZK, Chai HK, Cheng N, Yang Y, Wang DN, Yang T and Cao W, 2016. Melatonin treatment delays postharvest senescence and regulates reactive oxygen species metabolism in peach fruit. Postharvest Biology and Technology 118: 103–110.

Hodges DM, DeLong JM, Forney CF and Prange RK, 1999. Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207: 604–611.

Hu W, Tie W, Ou W, Yan Y, Kong H, Zuo J, Ding X, Ding Z, Liu Y and Wu C, 2018. Crosstalk between calcium and melatonin affects postharvest physiological deterioration and quality loss in cassava. Postharvest Biology and Technology 140: 42–49.

Jiang T, 2013. Effect of alginate coating on physicochemical and sensory qualities of button mushrooms (Agaricus bisporus) under a high oxygen modified atmosphere. Postharvest Biology and Technology 76: 91–97.

Jiang YM, Duan XW, Joyce D, Zhang ZQ and Li JR, 2004. Advances in understanding of enzymatic browning in harvested litchi fruit. Food Chemistry 88: 443–446.

Khan ZU, Aisikaer G, Khan RU, Bu J, Jiang Z, Ni Z and Ying T, 2014. Effects of composite chemical pretreatment on maintaining quality in button mushrooms (Agaricus bisporus) during postharvest storage. Postharvest Biology and Technology 95: 36-41.

Lagnika C, Zhang M and Mothibe KJ, 2013. Effects of ultrasound and high pressure argon on physico-chemical properties of white mushrooms (Agaricus bisporus) during postharvest storage. Postharvest Biology and Technology 82: 87–94.

Li L, Kitazawa H, Zhang X, Zhang L, Sun Y, Wang X and Yu S, 2021. Melatonin retards senescence via regulation of the electron leakage of postharvest white mushroom (Agaricus bisporus). Food Chemistry 340: 127833.‏

Liu C, Zheng H, Sheng K, Liu W and Zheng L, 2018. Effects of melatonin treatment on the postharvest quality of strawberry fruit. Postharvest Biology and Technology 139: 47–55.

Luo F, Cai JH, Zhang X, Tao DB, Zhou X, Zhou Q and Ji SJ, 2018. Effects of methyl jasmonate and melatonin treatments on the sensory quality and bioactive compounds of harvested broccoli. RSC advances 8(72): 41422-41431.‏

Ma Q, Zhang T, Zhang P and Wang ZY, 2016. Melatonin attenuates postharvest physiological deterioration of cassava storage roots. Journal of Pineal Research 60(4): 424–434.

Marino B and Hernandez A, 2014. Melatonin: plant growth regulator and or biostimulator during stress. Journal of Plant Biology 19.

Meng D, Song T, Shen L, Zhang X and Sheng J, 2012. Postharvest application of methyl jasmonate for improving quality retention of Agaricus bisporus fruit bodies. Journal of Agriculture of Food Chemistry 60(23): 6056-6062.

Nasiri M, Barzegar M, Sahari MA and Niakousari M, 2017. Tragacanth gum containing Zataria multiflora Boiss. Essential oil as a natural preservative for storage of button mushrooms (Agaricus bisporus). Food Hydrocolloids 72 (Supplement C): 202–209.

Nguyen T, Ketsa S and van Doorn WG, 2003. Relationship between browning and the activities of polyphenoloxidase and phenylalanine ammonia lyase in banana peel during low temperature storage. Postharvest Biology and Technology 30(2): 187-193.

Oms-Oliu G, Aguiló-Aguayo I, Martín-Belloso O and Soliva-Fortuny R, 2010. Effects of pulsed light treatments on quality and antioxidant properties of fresh-cut mushrooms (Agaricus bisporus). Postharvest Biology and Technology 56(3): 216-222.‏

Rastegar S, Khankahdani HH and Rahimzadeh M, 2020. Effects of melatonin treatment on the biochemical changes and antioxidant enzyme activity of mango fruit during storage. Scientia Horticulturae 259: 108835

Shi H, Jiang C, Ye T, Tan DX, Reiter RJ, Zhang H, Liu R and Chan Z, 2015. Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of improved abiotic stress resistance in Bermuda grass [Cynodon dactylon (L). Pers.] By exogenous melatonin. Journal of Experimental Botany 66: 681–694.

Shrivas K, Agrawal K, Patel DK and Kumar A, 2005. A spectrophotometric determination of ascorbic acid. Journal-chinese chemical society Taipei 52: 503.

Singleton VL and Rossi JA, 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture 16(3): 144-158.

Suttirak W and Manurakchinakorn S, 2010. Potential application of ascorbic acid, citric acid and oxalic acid for browning inhibition in fresh-cut fruits and vegetables. Walailak Journal of Science and Technology 7: 5-14.

Sun Q, Zhang N, Wang J, Cao Y, Li X, Zhang H, Zhang LY, Tan DX and Guo Y, 2016. A label-free differential proteomics analysis reveals the effect of melatonin on promoting fruit ripening and anthocyanin accumulation upon postharvest in tomato. Journal of Pineal Research 61: 138–153.

Tan DX, Manchester LC, Liu X, Rosales-Corral SA, Acuna-Castroviejo D and Reiter RJ, 2013. Mitochondria and chloroplasts as the original sites of melatonin synthesis: A hypothesis related to melatonin’s primary function and evolution in eukaryotes. Journal of Pineal Research 54: 127–138.

Terada M, Watanabe Y, Kunitomo M and Hayashi E, 1978. Differential rapid analysis of ascorbic acid and ascorbic acid 2-sulfate by dinitrophenylhydrazine method. Analytical Biochemistry 84(2): 604-608.

Tomas-Barberan FA, Gil MI, Cremin P, Waterhouse AL, Hess-Pierce B and Kader AL, 2001. HPLC-DAD-ESIMS analysis of phenolic compounds in nectarines, peaches and plums. Journal of Agricultural and Food Chemistry 49: 4748–4760.

Tsai CJ, Harding SA, Tschaplinshi TJ, Lindroth RL and Yuan Y, 2006. Genome-wide analysis of the structural genes regulating defense phenylpropanoid metabolism in Populus. New Phytol 172: 47–62.

Xing Y, Li X, Xu Q, Yun J, Lu Y and Tang Y, 2011. Effects of chitosan coating enriched with cinnamon oil on qualitative properties of sweet pepper (Capsicum annuum L.). Food Chemistry 124: 1443-1450.

Zhang N, Sun Q, Li H, Li X, Cao Y, Zhang H, Li S, Zhang L, Qi Y and Ren S, 2016. Melatonin improved anthocyanin accumulation by regulating gene expressions and resulted in high reactive oxygen species scavenging capacity in cabbage. Frontiers in Plant Science 7: 197.

Zhang H, Liu X, Chen T, Ji Y, Shi K, Wang L, Zheng X and Kong J, 2018. Melatonin in Apples and Juice: Inhibition of browning and microorganism growth in apple juice. Molecules 23: 521.

Zheng H, Liu W, Liu S, Liu C and Zheng L, 2019. Effects of melatonin treatment on the enzymatic browning and nutritional quality of fresh-cut pear fruit. Food chemistry 299: 125--116.‏

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Food Research Journal
Volume 32, Issue 3
June 2022
Pages 111-128

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