بررسی ویژگی های عملکردی پپتیدهای حاصل از هیدرولیز پروتئین آرد گندم سالم توسط پروتئاز موجود در گندم سن زده

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم و مهندسی صنایع غذایی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان، اصفهان، ایران

2 علوم و مهندسی صنایع غذایی، دانشکده کشاورزی،دانشگاه ایلام

3 گروه علوم و مهندسی صنایع غذایی- دانشکده کشاورزی- دانشگاه صنعتی اصفهان

چکیده

در این پژوهش، به بررسی تاثیر پروتئاز استخراجی از گندم سن زده ( سرین پروتئاز) بر هیدرولیز آرد گندم سالم و تولید پپتید های آزاد حاصل از هیدرولیز پرداخته شده است. هدف: هدف از این مطالعه تولید پپتید های کوتاه زنجیر با خواص عملکردی و درمانی مفید مانند آنتی اکسیدانی توسط پروتئاز گندم سن زده می باشد. روش کار: آزمون های مورد بررسی در این پژوهش، آزمون تعیین فعالیت آنزیمی، آزمون درجه هیدرولیز به روش شناساگر اورتوفتالدهید، خواص عملکردی مانند حلالیت، آنتی اکسیدانی و امولسیون کنندگی، ظرفیت نگهداری حلال (SRC) و در ادامه عکسبرداری با میکروسکوپ الکترونی (SEM) بودند. آنالیز آماری با استفاده از نرم افزار SPSS در سطح اطمینان 95 درصد انجام گرفت. نتایج: در این مطالعه مشاهده شد که فعالیت آنزیمی آرد گندم سن زده در مقایسه با آرد گندم سالم دارای تفاوت معناداری در سطح اطمینان 95 درصد بود. درجه هیدرولیز آرد گندم تیمار شده با عصاره آنزیمی آرد سن زده در مقایسه با نمونه شاهد ( آرد گندم سالم) افزایش پیدا کرد. در رابطه با طول پپتید نیز مشاهده شد که طول پیتید های ایجاد شده در آرد گندم سالم تیمار شده با عصاره آنزیمی سن زده بسیار کوتاه تر از طول پپتید های نمونه شاهد (آرد سالم) بود. با افزایش درجه هیدرولیز، خواص عملکردی مانند خاصیت آنتی اکسیدانی و حلالیت افزایش پیدا کرد اما ظرفیت امولسیون کنندگی روند نزولی داشت. نتایج حاصل از ظرفیت نگهداری حلال (SRC) حاکی از آن بود که با افزایش هیدرولیز، SRCدر حلال اسید لاکتیک کاهش پیدا کرد و در حلال آب بی تاثیر بود. در عکسبرداری با میکروسکوپ الکترونی (SEM) نیز مشاهده گردید که در گندم سن زده نسبت به گندم سالم ساختار پروتئینی تخریب گردیده و گویچه های پروتئینی تا حدودی از بین رفته اند. نتایج حاکی از آن بود که استفاده از پروتئاز عصاره آنزیمی سن زده در تولید پپتید های آزاد به خوبی عمل نموده است.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of functional properties of peptides obtained from hydrolysis of healthy wheat flour protein by sunn pest protease

نویسندگان [English]

  • mahsa yari 1
  • mohammadyar hosseini 2
  • mehdi kadivar 3
1 Department of Food Science and Engineering,, Faculty of Agriculture, Isfahan University of Technology, Isfahan,, Iran
2 Food technology, faculty of agriculture, Ilam University
3 Department of food science and engineering- faculty of agriculture- Isfahan university of technology- Iran
چکیده [English]

Introduction: Wheat is the most important sustainable food product for more than a third of the world's population and has more calories and protein than other cereal products in the world diet.
The main property that distinguishes it from other products is the unique properties of the resulting dough, which allows it to appear in a variety of breads and other food products such as cakes, biscuits and pasta. These properties in these products depend on the structures and interactions of the grain storage proteins, which together constitute the gluten protein. Lutein is the predominant carotenoid in wheat. Wheat bran and sprouts contain a lot of carotenoids and antioxidant activity compared to endosperm. Lutein along with zeaxanthin is important for human skin and eye health. Heart disease protection may come from whole grains, antioxidants, vitamins, and fiber and minerals. Whole grains are also effective in preventing diabetes. Whole grains appear to protect against heart disease and cancer. Proteins in plant tissue are simple and consist of four main types, including: albumin (soluble in water and dilute buffer), globulins (soluble in salt water) and prolamine (soluble in 90-90% ethanol) and glutlin (soluble in Wheat gluten protein is classically composed of two parts: alcohol-soluble gliadin and alcohol-insoluble glutenin. Glutenins are known as the largest polymers in nature. Wheat proteins can be divided into structural proteins (gluten-free) and storage proteins or (gluten). Structural proteins include albumin, globulin, and amphiphilic. Non-membrane amphiphilic proteins have many effects on grain hardness and rheological properties of dough. Wheat storage proteins are known as prolamins due to their amino acids proline and glutamine. Aqueous salivary secretions act rapidly on the plant cell, first increasing cell respiration and then causing the protoplasm to flow, which is usually due to increased permeability of the cell membrane. They contain soluble amino acids that immediately after secretion on plant materials with the formation of a large number of hydrogen bonds as well as a number of disulfide bonds in the form of gels that gradually become solid. In this study, the effect of extracted protease from aged wheat, protease (serine) has been investigated. The aim of this study was to produce short chain peptides with beneficial functional and therapeutic properties such as antioxidant, antidiabetic, anti-hypertension by aged wheat protease.
Material and Methods: The tests include determination of enzymatic activity, degree of hydrolysi, determination of functional properties such as solubility, antioxidant, emulsification, and solvent retention capacity (SRC). Microstructure of the contaminated seed was also investigated by electron microscopy (SEM) ). Statistical analysis was performed using SPSS software at 95% confidence level. First, for extraction of aged wheat flour mix 2 gr of flour with 10 cc of 0.2 M acetate buffer with pH = 3.8 and centrifuge for 5 minutes. The supernatant was separated and filtered from a 0.45 microfilter and stored in the freezer. flour enzymatic activity was performed in the first, 5 cc of 0.75% casein solution in which 50 mM hydrodisodium phosphate buffer with pH= 7 is equilibrated for 10 minutes at 37 ° C by pH. Slow addition of 0.1 N hydrochloric acid is adjusted to this substrate. To this substrate a certain volume of enzyme is diluted with 1 cc of active buffer of 30 mM cysteine hydrochloride monohydrate in 6 mM EDTA and diluted in a bath for 10 minutes and water 37 °C is mixed gently. The reaction is then stopped by adding 5 cc of 30% v / v trichloroacetic acid. At room temperature, cool with Whatman 42 filter paper and measure the absorption at 280 nm visible-ultraviolet wavelengths . For hydrolyse degree, First, make an opa reagent that dissolves 3.81 g of disodium tetra borate + 0.1 g of SDS plus 75 cc of distilled water, then dissolve 80 mg of orthophthaldehyde in 2 cc of 96% ethanol and place it on the shaker with a magnet until to be solved. Add 250 microliters of mercaptoethanol to the main container and then increase the volume to 100 with distilled water. The next step is to add 0.5 g of flour in different proportions with the same enzymatic activity plus 10 cc of distilled water and in the control sample we used water instead of enzyme. Then we put the tubes on the shaker for 30 minutes and then centrifuge for 20 minutes at 10,000 rpm and 4 ° C and add 400 μl to 3 ml of opa and then zero with reagent and then absorb at 340 nm. we read.Also, the effect of this protease enzyme on functional properties and creation of free peptides was investigated.
Results and discussion: It was observed that the enzyme extracted from aged wheat performed better in the creation of free peptides and increased the functional properties such as antioxidant properties and solubility, but the emulsifying properties of healthy flour from flour with aged enzymatic extract increased due to lower hydrolysis of healthy flour than healthy flour with aged enzyme. Wheat SRC was carried out according to AACC no. 11-56 method with two solvents of water and lactic acid, the results of which indicated that by increasing hydrolysis, SRC decreased in lactic acid solvent and was ineffective in water solvent. In addition, electron microscopy (SEM) with a magnification of 100 and 50 μm was observed that in aged wheat, protein structure was degraded compared to healthy wheat and protein cells were partially destroyed. These peptides are very beneficial for human health and are used in food industry and various food products.
Conclusion: The results of this study showed that the use of protease enzyme of sunn pest flour wheat enzyme extract in the production of free peptides has worked well and also has been very effective in improving functional properties such as solubility, antioxidants. Continuous innovations in the food industry and their higher quality requirements force the food industry to produce flours with specific functional properties. It seems protease isolated from sunn pest wheat flour might be considered as a suitable source for creating peptides with several advantages. According to the results, it was observed that aged wheat protease is a suitable animal protease for peptides with beneficial health benefits. In this study, it was found that in general, the advantages of age protease are the cheapness and availability of aged wheat and its high hydrolysis.

کلیدواژه‌ها [English]

  • Serine protease
  • Bioactive peptide
  • Functional property
  • Solvent retention capacity
کیومرثی م، کدیور م، زارعی س و طالبی م، 2017. بررسی خواص کیفی، عملکردی و زیست‌فعالی در گندم سن‌زده. پژوهش و نوآوری در علوم و صنایع غذایی, 5(4), 394-383.
نجفی میرک ت، نجفیان گ، خرسندی ه، معین نمینی س و شرفی گ، 1392. اثر سن زدگی دانه بر کیفیت نانوایی ارقام گندم نان. مجله به زراعی نهال و بذر (نهال و بذر)، 29(2), 427-413.
Akić, S, Petrović S, Kukić J, Jadranin M, Tešević V, Povrenović D and Šiler-Marinković S, 2007. Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acornsom Serbia. Food Chemistry 104(2): 830-834.
Alcin E, Sakiyan O, Sumnu G, Celik S and Koksel H, 2008 . Functional properties of microwave- w treated heat gluten. European Food Research and Technology 227(5): 1411-1417.
Aluko R and Monu E, 2003. Functional and bioactive properties of quinoa seed protein hydrolysates. Journal Food Science 68:1254-1258.
Amza T, Balla A, Tounkara F, Man L and Zhou HM, 2013. Effect of hydrolysis time on nutritional, functional and antioxidant properties of protein hydrolysates prepared from gingerbread plum (Neocarya macrophylla) seeds. International Food Research Journal 20(5): 2081-2090.
Bonet A, Caballero PA, Gómez M and Rosell CM, 2005. Microbial transglutaminase as a tool to restore the functionality of gluten from insect damaged wheat. Cereal Chemistry 82(4): 425-430.
Bubler S, Rumpold BA, Jander E, Rawel HM and Schlüter OK, 2016 . Recovery and techno-functionality of flours and proteins from two edible insect species: Meal worm (Tenebrio molitor) and black soldier fly (Hermetia illucens) larvae. Heliyon 2(12): 1-23.
Englund PT, King TP, Craig LC and Walti ANDA, 1968 . Ficin. Its isolation and characterization. Biochemistry 7(1): 163-175.
Korhonen H and Pihlanto A, 2006. Bioactive peptides: production and functionality. International Dairy Journal 16(9): 945-960.
Li X, Xiong H, Yang K, Peng D, Peng H and Zhao Q, 2012 . Optimization of the biological processing of rice dregs into nutritional peptides with the aid of trypsin. Journal of food science and technology 49(5): 537-546.
Oladele AK and Aina JO, 2007 . Chemical composition and functional properties of flour produced from two varieties of tigernut (Cyperus esculentus). African Journal of Biotechnology 6(21): 1-10.
Pourmohammadi K and Abedi E, 2021. Hydrolytic enzymes and their directly and indirectly effects on gluten and dough properties: An extensive review. Food Science & Nutrition 437-449.
Ram S, Dawar V, Singh RP and Shoran J, 2005. Application of solvent retention capacity tests for the prediction of mixing properties of wheat flour. Journal of Cereal Science 42(2): 261-266.
 Salas CE, Dittz D and Torres MJ, 2018 . Plant proteolytic enzymes: Their role as natural pharmacophores. In Biotechnological applications of plant proteolytic enzymes Springer. Cham 107-127.
Torbica AM, Mastilović JS, Pojić MM and Kevrešan ŽS, 2014 . Effects of wheat bug (Eurygaster spp and Aelia spp) infestation in preharvest period on wheat technological quality and gluten composition. The
Scientific World Journal 232-241.
Wang CC and Grant DR, 1969. The proteolytic enzymes in wheat flour. Cereal Chemistry 46: 537-544.