اثر غلظت‌های مختلف لستین- کلسترول بر پایداری درونپوشانی نانولیپوزوم‌های حامل بتاکاروتن

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم و صنایع غذایی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل

چکیده

هدف از این تحقیق بررسی تاثیر نسبت‌های متفاوت لسیتین-کلسترول بر اندازه ذرات، درون‌پوشانی و پایداری این دو ویژگی در طول زمان است. همچنین بررسی کارایی روش ترکیبی هیدراسیون لایه نازک-سونیکاسیون برای رفع مشکلات بزرگی اندازه ذرات و توزیع غیریکنواخت اندازه ذرات است. روش کار: تیمارهایی آزمایشی در این پژوهش شامل نسبت‌های ترکیبی لسیتین-کلسترول ( 30-30، 40-20، 50-10 و 60-0) می‌باشد. با توجه به عدم امتزاج‌پذیری بتاکاروتن با آب، فرمولاسیون‌های نانولیپوزوم‌ها با استفاده از روش ترکیبی هیدراسیون لایة نازک- سونیکاسیون تولید گردید. برای کاهش اندازه ذرات، مخلوط لیپوزومی به داخل حمام یخ (به منظور اجتناب از اعمال انرژی مازاد به داخل محلول و پیش‌‌گیری از هیدرولیز و اکسیداسیون لیپید) و سونیکاتور پروب منتقل شد و 10 سیکل 1 دقیقه‌ای با 1 دقیقه استراحت ما بین سیکل‌ها به مخلوط اعمال شد. نتایج: نتایج نشان داد که اثر غلظت‌های ترکیبی لسیتین-کلسترول بر تغییرات اندازه ذارت، توزیع اندازه (PDI) ذرات، بازده درون‌پوشانی و پتانسیل زتا معنی‌دار است. با افزودن کلسترول به نسبت 5:1 به لسیتین اندازه ذرات و توزیع اندازه ذرات کاهش و بازده درون‌پوشانی افزایش یافت، اما با افزایش بیشتر نسبت کلسترول به لسیتین اندازه ذرات و توزیع اندازه ذرات افزایش و بازده درون‌پوشانی کاهش یافت. همچنین پتانسیل زتای تمام نمونه‌ها در محدوده مطلوبی قرار داشت و با افزایش کلسترول اندازه پتانسیل زتا افزایش یافت. همچنین نتایج این تحقیق نشان داد که افزودن کلسترول به نسبت 5:1 به لسیتین در ساختار نانولیپوزوم باعث می‌شود که در بازه زمانی 60 روز اندازه ذرات و کارایی درون‌پوشانی بتاکاروتن نسبت به ساختار نانولیپوزوم فاقد کلسترول پایداری بیشتری داشته باشد. همچنین آزمون FTIR مشخص کرد که ذرات بتاکاروتن به خوبی درون ساختار نانولیپوزوم‌ها به دام افتاده است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of different ratios of lecithin-cholesterol on the encapsulation stability of beta-carotene loaded nanoliposomes

نویسندگان [English]

  • Bahram Fathi-Achachlouei
  • Sahar Namvar
  • Rezvan Shaddel
University of Mohaghegh Ardabili
چکیده [English]

Introduction:
Encapsulation using nanoliposomes is widely recognized as an effective technique to protect sensitive bioactive compounds from adverse processing or gastrointestinal conditions. The physical properties of liposome (especially in terms of size, size distribution and layering) depend on different preparation methods, type of lipid (charged, uncharged and neutral), lipid composition, surfactant, organic solvent and ionic strength of the suspension medium used in the preparation techniques. The common method used to produce liposome is thin layer hydration. The application of this method is limited due to the large particle size and non-uniform particle size distribution. The purpose of this research is to investigate the effect of different ratios of lecithin-cholesterol on particle size, encapsulation efficiency and stability of these two characteristics over time. Also, another goal of this research is to investigate the effectiveness of the combined method of thin layer hydration and sonication to solve the problems of large particle size and non-uniform distribution of particle size.
Material & methods: Experimental treatments in this research include lecithin-cholesterol ratios (30-30, 40-20, 50-10 and 60-0). Due to the incompatibility of beta-carotene with water, nanoliposome formulations were produced using the combined method of thin layer hydration-sonication. Subsequently, to reduce the size of the particles, the liposomal mixture was transferred to the ice bath (in order to avoid applying excess energy into the solution and to prevent lipid hydrolysis and oxidation) and probe sonicator and 10 cycles of 1 minute with 1 minute rest between cycles were applied to the mixture.

Results and discussion: The results showed that the effect of lecithin-cholesterol ratios on changes in particle size, particle size distribution (PDI), encapsulation efficiency and zeta potential is significant. By adding cholesterol at ratio of 1:5 to lecithin, the particle size and particle size distribution decreased and the encapsulation efficiency increased; however, with a further increase in the ratio of cholesterol to lecithin, the particle size and particle size distribution increased and the encapsulation efficiency decreased. Also, the zeta potential of all the samples was within the desired range, and by the increase in cholesterol, the zeta potential increased. Also, the results of this research showed that the addition of cholesterol at a ratio of 1:5 to lecithin in the nanoliposome structure makes the particle size and encapsulation efficiency of beta-carotene more stable in 60 days compared to the nanoliposome structure without cholesterol. Also, FTIR test determined that beta-carotene particles are well trapped in the structure of nanoliposomes.
Conclusion: According to the tests examined in this research, it can be concluded that the combined method of thin layer hydration-sonication along with the limited use of cholesterol is a suitable solution to obtain nano-scale particles with high stability over time.
Introduction:
Encapsulation using nanoliposomes is widely recognized as an effective technique to protect sensitive bioactive compounds from adverse processing or gastrointestinal conditions. The physical properties of liposome (especially in terms of size, size distribution and layering) depend on different preparation methods, type of lipid (charged, uncharged and neutral), lipid composition, surfactant, organic solvent and ionic strength of the suspension medium used in the preparation techniques. The common method used to produce liposome is thin layer hydration. The application of this method is limited due to the large particle size and non-uniform particle size distribution. The purpose of this research is to investigate the effect of different ratios of lecithin-cholesterol on particle size, encapsulation efficiency and stability of these two characteristics over time. Also, another goal of this research is to investigate the effectiveness of the combined method of thin layer hydration and sonication to solve the problems of large particle size and non-uniform distribution of particle size.
Material & methods: Experimental treatments in this research include lecithin-cholesterol ratios (30-30, 40-20, 50-10 and 60-0). Due to the incompatibility of beta-carotene with water, nanoliposome formulations were produced using the combined method of thin layer hydration-sonication. Subsequently, to reduce the size of the particles, the liposomal mixture was transferred to the ice bath (in order to avoid applying excess energy into the solution and to prevent lipid hydrolysis and oxidation) and probe sonicator and 10 cycles of 1 minute with 1 minute rest between cycles were applied to the mixture.

Results and discussion: The results showed that the effect of lecithin-cholesterol ratios on changes in particle size, particle size distribution (PDI), encapsulation efficiency and zeta potential is significant. By adding cholesterol at ratio of 1:5 to lecithin, the particle size and particle size distribution decreased and the encapsulation efficiency increased; however, with a further increase in the ratio of cholesterol to lecithin, the particle size and particle size distribution increased and the encapsulation efficiency decreased. Also, the zeta potential of all the samples was within the desired range, and by the increase in cholesterol, the zeta potential increased. Also, the results of this research showed that the addition of cholesterol at a ratio of 1:5 to lecithin in the nanoliposome structure makes the particle size and encapsulation efficiency of beta-carotene more stable in 60 days compared to the nanoliposome structure without cholesterol. Also, FTIR test determined that beta-carotene particles are well trapped in the structure of nanoliposomes.
Conclusion: According to the tests examined in this research, it can be concluded that the combined method of thin layer hydration-sonication along with the limited use of cholesterol is a suitable solution to obtain nano-scale particles with high stability over time.
Keywords: Beta-carotene, nanoliposome, thin film hydration, sonication, lecithin, cholesterol

کلیدواژه‌ها [English]

  • Beta-carotene
  • nanoliposome
  • thin film hydration
  • sonication
  • lecithin
  • cholesterol

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پژوهش های صنایع غذایی
دوره 33، شماره 4
اسفند 1402
صفحه 131-149

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