ارزیابی اثر زمان برداشت و مدت نگهداری میوه در سردخانه بر روی برخی خصوصیات کیفی میوه زغال اخته

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تبریز

چکیده

زمینه مطالعاتی: ارزیابی خواص فیزیکوشیمیایی میوه­ها در طی مراحل مختلف بلوغ، برای دستیابی به محصول با کیفیت بالا و گسترش طول دوره انبارمانی ضروری می­باشد. هدف: بررسی تأثیر زمان برداشت بر خواص فیزیکی­شیمیایی، ویتامین ث و کیفیت میوه­های زغال اخته در طول دوره انبارمانی بود. روش کار: در این پژوهش اثر زمان برداشت (برداشت اول در مرحله برداشت تجاری و برداشت دوم به فاصله 5 روز بعد از برداشت اول) و مدت نگهداری میوه در سردخانه بر برخی ویژگی­های فیزیکوشیمیایی  میوه زغال­اخته در دو مرحله زمان برداشت انجام شد. میوه‌ها به سردخانه با دمای°C 4 و رطوبت نسبی 85-80٪ به مدت 21 روز منتقل شدند و صفات کیفی از قبیل محتوای pH، مواد جامد محلول کل (TSS)، اسیدیته کل (TA)، نسبت TSS/TA، ویتامین ث، میزان نشت یونی و تولید اتیلن میوه­ها در طول دوره انبارمانی (زمان صفر انبارمانی (0)، 7، 14 و 21 روز پس از انبارمانی) مورد ارزیابی قرار گرفتند. نتایج: تاخیر در برداشت تاثیر معنی­داری بر میزان pH و نشت یونی میوه­ها نداشت، اما سبب افزایش (01/0 P<)  نسبت مواد جامد محلول به اسیدیته قابل تیتراسیون (TSS/TA)  و کاهش میزان اسیدیته قابل تیتراسیون  (TA) میوه گردید. از نظر طول دوره نگهداری در انبار در تمام صفات مورد بررسی اختلاف معنی­داری (01/0 P<) وجود داشت. به­طوری که با پیشرفت مرحله بلوغ و رسیدگی میوه در طول دوره انبارمانی، میزان مواد جامد محلول کل (TSS) و تولید اتیلن در میوه­های برداشت دوم با افزایش طول دوره نگهداری در انبار نسبت به برداشت اول روند افزایشی نشان دادند، درحالی­که محتوای ویتامین ث برداشت اول روند کاهشی بیشتری نشان داد. نتیجه گیری نهایی: تأخیر در برداشت، سبب افزایش مواد جامد محلول کل (به عنوان یک پارامتر کیفی مهم می‌باشد که رابطه مستقیم با کیفیت خوراکی میوه در زمان رسیدن دارد) و کاهش اسیدیته میوه گردید که در نتیجه نسبت قند به اسید افزایش یافته و سبب افزایش کیفیت میوه‌ها ­گردید. همچنین تأخیر در برداشت سبب افزایش ویتامین ث میوه، که به عنوان یک آنتی­اکسیدان طبیعی در گیاه می­باشد، شد. ولی زمان برداشت تأثیری بر میزان pH و نشت یونی نداشت.با گذشت مدت زمان انبارمانی، میزان pH، نسبت مواد جامد محلول کل به اسیدیته کل و نشت یونی میوه­ها افزایش یافتند، در حالی که محتوای اسیدیته کل کاهش یافت.         

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the effect of harvest time and fruit cold storage period on some of qualitative characteristics of Cornelian cherry fruit

نویسندگان [English]

  • N Esmaeili
  • R Naghshband
  • F Zare Nahandi
چکیده [English]

Introduction: Evaluation of the physicochemical properties of fruits during different stages of maturity is essential for achieving high-quality product and extending shelf life. Harvest maturity and storage time are main factors that may lead to changes in sensory and nutritional qualities of cornelian cherries. Cornelian cherry fruit are frequently harvested at dark red stages, when their flavor is most desirable. Consumers do not usually eat cornelian cherry at any of the other maturation stages. Therefore, the effect of ripening and storage time on nutritional quality is a major issue. The cornelian cherry fruits which have sour and sweat tasting juice, contain a high amount of vitamin C. To optimum threshing performance, processes of pnematic conveying, storing and other processes of cornelian cherry fruits, its physical properties should be known.  
Materials and methods: This research was conducted to investigate the effect of harvest time at two stages (commercial harvest and 5 days after the first harvest) on some of the physicochemical properties of a commercial genotype of cornelian cherry fruit during the storage period. The fruit kept in cold storage at a temperature of 4 °C with relative humidity of 80-85% for 21 days. Fruit qualitative traits such as pH content, total soluble solids (TSS), titrable acidity (TA), TSS / TA ratio, vitamin C, ion leakage and ethylene production of fruits during storage at 0, 7, 14 and 21 days of storage were evaluated. Total soluble solids were determined by a hand refractometer (model Atago N, Japan) having range of 0-32 percent and the values obtained in per cent were correlated at 20ºC. Titratable acidity was determined by taking a known weight of fruit juice and making a known volume of it by adding distilled water. Then a known volume of this liquid was treated against 0.1 N sodium hydroxide at pH 8.2 as an indicator of titration using pH meter. The ascorbic acid (vitamin C) content in the aqueous supernatant of each maturity stage was determined.
 Results and discussion: The results showed that the harvest time has not a significant effect on pH and ion leakage of fruits. The delay in the harvest time increased the total solids soluble solids to total acidity ratio (TSS / TA) ratio and decreased total acidity (TA). However, there was a significant difference during storage period in all of the studied parameters. With progress in the maturity stage and fruit during the storage period, total soluble solids (TSS) and ethylene production in second harvest fruits showed an increase comparing the first harvest time. The increase in soluble solids content of fruit during storage period could be related to conversion of insoluble carbohydrates to soluble ones and decreasing of transpiration of fruit during storage period (Miaruddin et al. 2011). pH, total soluble solids to total acidity ratio (TSS / TA), and ion leakage of fruits increased, while the total acidity content decreased along with storage time. During ripening period of strawberry, the content of organic acids in fruit reduced and soluble sugars increased which led to increasing soluble solids content to total acidity ratio and sweeter fruit taste (Winardiantica et al. 2015). Foliar application of salicylic acid with high concentrations (2 mM) increased fruits phenol content, vitamin C, TA and total anthocyanin compound. Maximum amount of total antioxidant has been established in 2mM SA concentration that demonstrated 18% increase in compare with control. SA foliar application caused to decrease of total soluble solid (TSS) content but had not any significant effects on flavonoid and total carotenoid of grape berries. These results further indicated that the effects of Salicylic acid in grape is associated with induce defensive systems and increase biological performance such as antioxidant activity and different quality fields in grape (Ghohari et al. 2018). The content of vitamin C (ascorbic acid) of the first harvest had lower than second harvest and showed a declining trend for both of them during storage period. Many pre- and postharvest factors influence the vitamin C content of horticultural crops. Large genotypic variation in vitamin content, climatic conditions and cultural practices, Maturity at harvest, harvesting method, and postharvest handling conditions also affect the vitamin C content of fruits and vegetable (Gordon et al 2012). Chaudhari et al. (2017) reported that the ascorbic acid content of citrus fruit decreased during storage period which may related to chilling injury incidence of fruits. Membrane ion leakage of cornelian cherry fruit increased by the end of storage period which led to water soaked fruits. By the end of storage at cold temperature membrane leakage percent of both harvests increased. This may be caused by cold stress oxidative injury effect on fruit cells and softens their texture. Antioxidant activity was high in fruits and varied greatly among the genotypes. So cornelian cherry could be considered a good source of natural antioxidants. They can potentially be used in food and nutraceutical supplement formulations as well. Fruit weight, soluble solids content and acidity were varied significantly among genotypes.  Shewfelt and Purvis (1995) demonstrated that membrane ion leakage in plants could be an indirect signal of membrane integrity state which decreased by the chilling injury incidence in fruit during cold storage period. During storage period, ethylene production of both harvested fruit were increased as the values for first harvest were lower than second harvest. The increase in ethylene production may be related to the effect of cold temperature stress during storage period and change the fruit metabolism as pheylpropanoid pathway (Yung and Hoffman 1984). Ripening and senescence involve the last phase of molecular and biochemical changes that result in the transformation of fruits into an edible form with aesthetically superior organoleptic and nutritional qualities. These changes are initiated in response to ethylene.  
Conclusion: Generally, our results showed that with delay in harvest soluble solid content of fruit increased and total acidity of fruit decreased. Also, fruit of second harvest had more ascorbic acid content than first harvest that a good qualitative index as an antioxidant compound in cornelian cherry fuit for having a good quality fruit by the end of storage period. So the second harvest fruit was more qualitative than first harvest during storage period.

اثنی ­عشری م، زکایی خسروشاهی م.ر، 1390. فیزیولوژی و تکنولوژی پس از برداشت. چاپ دوم، انتشارات دانشگاه بو علی سینا، 658 صفحه.
عشورنژاد م، قاسم­نژاد م، آقاجان­زاده س، فتاحی­مقدم ج و بخشی د، 1389.  ارزیابی عمر انباری و کیفیت پس از برداشت میوه­های کیوی رقم ’هایوارد‘ تولید شده در سیستم­های کشاورزی ارگانیک و متداول. مجله دانش کشاورزی و تولید پایدار، دوره 22، شماره 3. صفحه­های 12-1.
فتاحی­مقدم ج، حلاجی­ثانی م، 1391. تعیین زمان مناسب برداشت میوه کیوی و تأثیر آن بر کیفیت میوه پس از برداشت. مجله علوم باغبانی. دوره 26، شماره2. صفحه­های 237-230.
قاسم­نژاد م، قربانعلی­پور و فتاحی­مقدم ج، 1390. تأثیر زمان برداشت بر ظرفیت آنتی­اکسیدانی و کیفیت نگهداری میوه کیوی رقم هایوارد. مجله به­زراعی کشاورزی، دوره 13، شماره 1. صفحه­های 64-55.
گوهری غ، صفایی ف، رسولی ف، اعظمی م، دواتی کاظم نیا ح، 1397. ارزیابی اثرات محلول پاشی اسید سالسیلیک بر فعالیت برخی ترکیبات آنتی اکسیدانی انگور رقم  شاهانیVitis Vinifera L. cv Shahani) (. نشریه پژوهشهای صنایع غذایی، جلد 28، شماره 2.  صفحه های 159- 149.
مشرف ل، قاسمی ا، 1383. اثر زمان برداشت بر افزایش عمر نگهداری بِه رقم اصفهان. نشریه علوم آب و خاک، دوره 8، شماره 2. صفحه­های 190-181.
Ackermann J, Fischer M and Amadò R, 1992. Changes in Sugars, Acids, and Amino Acids during Ripening and Storage of Apples (cv. Glockenapfel). Journal of Agricultural and Food Chemistry 40(7): 1131–1134.
Bijelić S, Gološin B, Todorović JN and Cerović S, 2010. Morphological characteristics of best Cornelian cherry (Cornus mas L.) genotypes selected in Serbia. Genetic Resources and Crop Evolution 58: 689-695.
Boquete EJ, Trinchero GD, Fraschina AA, Vilella F and Sozzi GO, 2004. Ripening of “Hayward” kiwifruit treated with 1-methylcyclopropene after cold storage. Postharvest Biology and Technology 32(1): 57–65.Burg SP, Burg EA, 1962.The role of ethylene in fruit ripening. Plant Physiol 37:179–189.
Chase L, 1995. Quality counts: steps for top grade kiwifruit. California Grow Services, 19: 26-27.
Chaudhary PR, Yu X, Jayaprakasha GK and Patil BS, 2017. Influence of storage temperature and low-temperature conditioning on the levels of health-promoting compounds in Rio Red grapefruit. Food Science and Nutrition 5(3): 545–553.
Crisosto C, Kader A, 1999. Kiwifruit postharvest quality maintenance guidelines. Central Valley Postharvest Newsletter 8(3): 1-11.
Ercýslý S, 2004. Cornelian cherry germplasm resources of Turkey. Journal of Fruit and Ornamental Plant Research 92: 87-92.
Esteve MJ, Frígola A, Rodrigo C and Rodrigo D, 2005. Effect of storage period under variable conditions on the chemical and physical composition and colour of Spanish refrigerated orange juices. Food and Chemical Toxicology 43(9): 1413–1422.
Eyde RH, 1988. Comprehending Cornus: Puzzles and progress in the systematics of the dogwoods. The Botanical Review 54: 233-351.
Ganai SA, 2013. Effect of harvest dates and post-harvest treatments on quality and shelf-life of stored apple cv. red delicious, PhD Thesis, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir.
Gordon, A, Friedrich M, da Matta VM, Moura CFH and Marx F, (2012). Changes in phenolic composition, ascorbic acid and antioxidant capacity in cashew apple (Anacardium occidentale L.) during ripening. Fruits 67: 267-276.
Gunduz K, Saracoglu O, Özgen M and Serce S, 2013. Antioxidant, physical and chemical characteristics of cornelian cherry fruits (Cornus mas L.) at different stages of ripeness. ACTA Scientiarum Polonorum Horticulture 12: 59-66.
Hassanpour H, Yousef H, Jafar H and Mohammad A, 2011. Antioxidant capacity and phytochemical properties of cornelian cherry (Cornus mas L.) genotypes in Iran. Scientia Horticulturae 129(3): 459–463.
Horwitz W, Chichilo P and Reynolds H, (1970). Official methods of analysis of the Association of Official Analytical Chemists. Journal of Pharmaceutical Sciences 65(1): 162.
Jung SK, Watkins CB, 2008. Superficial scald control after delayed treatment of apple fruit with diphenylamine (DPA) and 1-methylcyclopropene (1-MCP). Postharvest Biology and Technology 50(1): 45–52. Klimenko S, 2004. The Cornelian cherry (Cornus mas L.) collection, preservation and utilization of genetic resources. J. Fruit Ornam. Plant Res. 12: 93-98.
Miaruddin M, Chowdhury MGF, Rahman MM, Khan MHH and Mozahid R, 2011. Effect of ripening chemicals on postharvest quality of tomato. Research Report (2010–2011) on Postharvest Technology of Crops. Postharvest Technogy Division Gazipur 1701: 79–85.
Moneruzzaman K, Hossain A, Sani W, Saifuddin M and Alenazi M, 2009. Effect of harvesting and storage conditions on the post-harvest quality of tomato (Lycopersicon esculentum Mill) cv. Roma VF. Australian Journal of Crop Science 3: 113-121.
Mussa S, Sharaa IEl, 2014. Analysis of Vitamin C (ascorbic acid) Contents packed fruit juice by UV-spectrophotometry and Redox Titration Methods. IOSR Journal of Applied Physics 6(5): 46–52.
Pantelidis G, Vasilakakis M, Manganaris G and Diamantidis G, 2007. Antioxidant capacity, phenol, anthocyanin and ascorbic acid contents in raspberries, blackberries, red currants, gooseberries and Cornelian cherries. Food chemistry 102: 777-783.
Pawlowska AM, Camangi F and Braca A, 2010. Quali-quantitative analysis of flavonoids of Cornus mas L. (Cornaceae) fruits. Food Chemistry 119: 1257-1261.
Pech, J, Balague C, Latche A and Bouzayen M, 1994. Postharvest physiology of climacteric fruits: recent developments in the biosynthesis and action of ethylene. Sciences des aliments 14: 3-15.
Peck GM, Andrews PK, Reganold JP and Fellman, JK, (2006). Apple Orchard Productivity and Food Quality under Organic, Conventional, and Integrated Management. HortScience 41(1): 99–107.
Piga, A, D'Aquino S and Agabbio M, 2000. Influence of cold storage and shelf-life on quality of 'Salustiana' orange fruits. Fruits (Paris) 55: 37-44.
Rab A, Rehman H, Haq I, Sajid M, Nawab K and Ali K, 2013. Harvest stages and pre-cooling influence the quality and storage life of tomato fruit. Journal of Animal and Plant Sciences 23(5): 1347–1352.
Rahaman AA, Bishop C, 2013. Evaluating the effects of biodegradable and conventional modified atmosphere packaging on the shelf life of organic Cavendish bananas. Journal of Post-Harvest Technology 1:29-35.
Sairam RK, Deshmukh PS and Shukla DS, 1997. Tolerance of Drought and Temperature Stress in Relation to Increased Antioxidant Enzyme Activity in Wheat. Journal of Agronomy and Crop Science 178(3): 171–178.
Seeram NP, Schutzki R, Chandra A and Nair MG, 2002. Characterization, quantification, and bioactivities of anthocyanins in Cornus species. Journal of Agricultural and Food Chemistry, 50, 2519-2523.
Shewfelt RL and Purvis AC, 1995. Toward a comprehensive model for lipid peroxidation in plant tissue disorders. HortScience 30: 213-218.
Smirnoff N, 1995. Antioxidant systems and plant response to the environment. Environment and plant metabolism: Flexibility and acclimation. Bios Scientific Publishers.
Winardiantika V, Lee YH, Park NI and Yeoung YR, 2015. Effects of cultivar and harvest time on the contents of antioxidant phytochemicals in strawberry fruits. Horticulture Environment and Biotechnology 56(6): 732–739.
Yang SF, Hoffmanne E, 1984. Ethylene biosynthesis and its regulation in high plant. Annual Review of Plant Physiology 35: 155 – 156.