بررسی تاثیر پوشش نانو امولسیون کیتوزان حاوی اسانس زوفا (Hyssopus officinalis) بر پدیده‌ی ملانوزیس در میگوی نگهداری شده در شرایط سرد

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه بهداشت مواد غذایی و آبزیان، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران

2 گروه تغذیه، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران

3 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

زمینه مطالعاتی: بهینه یابی پوشش نانوامولسیون کیتوزان حاوی اسانس زوفا باعث بهبود عملکرد این گروه از پوشش ها می گردد. هدف: در این مطالعه اثرات پوشش نانوامولسیون کیتوزان حاوی اسانس زوفا روی پدیده‌ی ملانوزیس و ارزیابی رنگ قطعات میگو طی 12 روز در 4 درجه سانتی گراد بررسی شد. روش کار: ابتدا اسانس زوفا به روش تقطیر با آب استخراج شد؛ اجزای تشکیل دهنده اسانس به وسیله GC/MS شناسایی شد. بیشترین ترکیبات آن شامل ایزوپینوکامفن (%45/35)، پینوکامفن (%81/11) و بتا-پاینن (%12/10) می باشد. این مطالعه شامل دو مرحله‌ی تهیه‌ی پوشش و مدل سازی غذایی می‌باشد. پس از تهیه‌ی نانوامولسیون کیتوزان حاوی اسانس، پارتیکل سایز و PDI تعیین شد. سپس نمونه ها در 10 تیمار آماده و برای ارزیابی رنگ و تعیین میزان ملانوزیس آنالیز شدند. نتایج: در روز 12 کمترین میزان قرمزی و زردی در گروه‌های نانوامولسیون کیتوزان حاوی اسانس زوفا (1% و %5/0) در مقایسه با گروه کنترل مشاهده شد. میزان روشنایی نمونه‌ها با افزایش زمان نگهداری در تمامی تیمار ها کاهش یافت؛ یکی از دلایل این کاهش به دلیل ظهور لکه های سیاه است. تیمار نانوامولسیون کیتوزان حاوی زوفا %1، بالاترین امتیاز و تیمار‌های کنترل، کیتوزان و کیتوزان سونیکیت شده پایین‌ترین امتیاز را به لحاظ ملانوزیس تا انتهای دوره در مقایسه با کنترل داشتند. نتیجه گیری: بر اساس نتایج این مطالعه پوشش نانوامولسیون کیتوزان حاوی اسانس زوفا می‌تواند باعث حفظ شاخص رنگ در گوشت میگو در مقایسه با گروه کنترل شود و رنگ آن را بهبود بخشد و به عنوان یک ترکیب ضد ملانوزیس جایگزین ترکیبات سنتزی مانند متا بی‌سولفیت سدیم شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effects of chitosan nanoemulsion coating containing Hyssopus officinalis essential oil on melanosis phenomenon in chill stored shrimp samples

نویسندگان [English]

  • saeid khanzadi 1
  • Abbas Mehraee 1
  • Mohammad Hashemi 2
  • Mohammad Azizzadeh 3
1 Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2 Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

Abstract

Introduction: Shrimp are highly perishable due to the biochemical, microbiological or physical changes during Post-mortem storage, which results in limited shelf life of the product (Farajzadeh et al., 2016). Melanosis is considered a limiting factor for crustacean preservation. This alteration originates by the action of polyphenol oxidase and in some species, as deep-water rose shrimp, also by the action of activated hemocyanin (Martínez-Alvarez et al., 2020). Traditional methods for shrimp preservation such as cold storage, freezing and chilling can’t suppress effectively spoilage (Farajzadeh et al., 2016). Hyssopus officinalis, as valuable medicinal herb, is widely used in traditional medicine. Due to the increased resistance of pathogenic microorganisms to antibiotics and increasing of treatment costs, attentions has been focused to compounds of natural origin (Pirnia et al., 1399). Nano-applications are named as one of the novel methods, which provide high immobilization efficiency for essential oils (keykhosravy e al., 2020). The nanoemulsion is a stable delivery system with unique physicochemical and practical characteristics including high physical stability, optical transparency and high bioavailability (khanzadi et al., 2020). In this study, the effects of chitosan nanoemulsion coating containing Hyssopus officinalis essential oil were investigated on melanosis phenomenon and color evaluation of shrimp samples during 12 days at 4 °C.
Material and methods: First, Hyssopus officinalis essential oil was prepared; Hyssop oil was extracted separately by water distillation. The essential oils components were identified by GC/MS (Pirnia et al., 1399). This study includes two phases of edible coating preparation and food modeling. After preparation of chitosan nanoemulsion containing essential oil, particle size and PDI were determined (keykhosravy e al., 2020). A dynamic light scattering (DLS) device (Malvern Instruments Ltd., United Kingdom) was utilized to measure the particle size of the nanoemulsion droplets. The distribution of droplet size was considered in the terms of the mean droplet size (z-diameter) and polydispersity index (PDI) (Moghimi et al., 2016). Then, Shrimp samples were provided and they were immediately placed in insulated polystyrene ice flasks and transported to the laboratory of food hygiene, Ferdowsi university of Mashhad, Mashhad, Iran. The fillets were washed completely for removing external particles (Mohajer et al., 2021). Shrimp samples were randomly divided into ten groups:
CON: without any coating solution
CH: Chitosan coating
SCH: Sonicated Chitosan coating
SMS: Sodium Metabisulfite coating
HEO 0.5%: Hyssopus officinalis essential oil (HEO) 0.5% (W/V)
HEO 1%: Hyssopus officinalis essential oil (HEO) 1% (W/V)
CE+HEO 0.5%: Chitosan coarse emulsion coating containing 0.5% (W/V) HEO
CE+HEO 1%: Chitosan coarse emulsion coating containing 1% (W/V) HEO
NE+HEO 0.5%: Chitosan nanoemulsion coating containing 0.5% (W/V) HEO
NE+HEO 1%: Chitosan nanoemulsion coating containing 1% (W/V) HEO
The prepared samples were covered with different solutions for 2 min and were allowed to drain for 1 h. All the samples were placed into zip packs. Lastly, the samples were kept at 4 ± 1 °C for 12 days and analyzed for color evaluation and melanosis during 2/4-day intervals (days 0, 2, 4, 8 and 12). Lightness (+L), yellowness (+b), and redness (+a) of samples were measured with a Hunterlab colorimeter (Hunter Associates Laboratory, Inc., Reston, Virginia, USA), using a CIELab scale (Salehi et al., 2018). Whiteness (W) was also calculated, as described by Park, 1994 (Park et., 1994). Melanosis or black spot of shrimp was performed based on ocular evaluations (Sani et al., 2017). First, the basic sensory evaluation techniques and characteristics of shrimp meat (taste, smell, color, and texture) were introduced to the members of the group. 21 evaluators (20-38 years) were selected from among the staff and students of the Food Hygiene Laboratory of the Faculty of Veterinary Medicine, Ferdowsi University of Mashhad. The evaluators evaluated the samples in individual panels with sufficient light and randomly. For each evaluator, drinking water was placed for the intervals between testing the samples (keykhosravy et al., 2021). Sensory evaluation was performed using a 4-point scoring technique. In this method, a score of four indicates the absence of black spots on the shrimp and a score of one indicates the presence of black spots across the surface of the shrimp.
Results and discussion: 33 compounds were identified in Hyssop. Most of its compounds include isopinocamphen (35.45%), pinocamphen (11.81%) and beta-pinene (10.12%). The results are in agreement with Najafpour et al. (2001) and Fraternale et al. (2004). The z-diameter and PDI values of CE+HEO 0.5% were determined to be 570.3 ± 15.83 nm and 0.63±0.07, while for CE+HEO 1% were 402.2 ± 2.50 nm and 0.50±0.05, respectively. Z-diameter of the NE+HEO 1% and NE+HEO 0.5% were measured as 385.7 ± 3.09 nm and 538.9 ± 4.40 nm, and their PDI values were 0.55±0.03 and 0.76±0.01, respectively. Color is the most important visual feature for consumers in seafood. A value is infinite and positive values are equivalent to red and negative values are equivalent to green. B values are infinite and positive values are equivalent to yellow and negative values are equivalent to blue, and L index is equal to the brightness of the image which is between 0 zero equivalent to black and 100 Equivalent to full reflection of light (Salehi et al., 2018). On day 12, the lowest amount of redness and yellowness was observed in the groups of chitosan nanoemulsion containing Hyssopus officinalis essential oil (1% and 0.5%) compared to the control group. The brightness of the samples decreased with increasing storage time in all treatments; one of the reasons for this decrease is due to the appearance of black spots. Chitosan nanoemulsion treatment containing Hyssopus officinalis 1% had the highest score and control, chitosan and sonicated chitosan treatments had the lowest score in terms of melanosis by the end of the period compared to the control.
Conclusion: According to the results of this study, chitosan nanoemulsion coating containing Hyssopus officinalis essential oil can maintain the color index in shrimp meat compared to the control group and improve its color and replace synthetic compounds such as sodium metabisulfite as an anti-melanosis compound.

کلیدواژه‌ها [English]

  • Chitosan
  • Nanoemulsion
  • Hyssopus officinalis
  • Melanosis
  • Black Spot
Acevedo-Fani A, Salvia-Trujillo L, Rojas-Graü MA and  Martín-Belloso O, 2015. Edible films from essential-oil-loaded nanoemulsions: Physicochemical characterization and antimicrobial properties. Food Hydrocolloids 47: 168-177.
Alparslan Y and Baygar T, 2017. Effect of chitosan film coating combined with orange peel essential oil on the shelf life of deepwater pink shrimp. Food and Bioprocess Technology 10 (5): 842-853.
Amaral AB, Silva MVd and Lannes SCd S, 2018. Lipid oxidation in meat: mechanisms and protective factors–a review. Food Science and Technology 38: 1-15.
Amiri E, Aminzare M, Azar HH and Mehrasbi MR, 2019. Combined antioxidant and sensory effects of corn starch films with nanoemulsion of Zataria multiflora essential oil fortified with cinnamaldehyde on fresh ground beef patties. Meat Science 153:66-74.
Batebi K, Latifi Z, Sepahvand S, Zare gashti Gh, Jamshidi M, Tehranian and Chaharlang M, 2022. Antioxidant effect of hydroalcoholic extract of peppermint on chemical changes and sensory properties of rainbow trout fillet (Oncorhynchus mykiss) at refrigerated temperature. Journal of Food Research 32 (4):149-166.
Bazargani-Gilani B, Aliakbarlu J and Tajik H, 2015. Effect of pomegranate juice dipping and chitosan coating enriched with Zataria multiflora Boiss essential oil on the shelf-life of chicken meat during refrigerated storage. Innovative Food Science & Emerging Technologies 29: 280-287.
Burt S, 2004. Essential oils: their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology 94(3): 223-253.
Donsì F, Annunziata M, Sessa M and Ferrari G, 2011. Nanoencapsulation of essential oils to enhance their antimicrobial activity in foods. LWT-Food Science and Technology 44(9): 1908-1914.
Farajzadeh F, Motamedzadegan A, Shahidi SA and Hamzeh S, 2016. The effect of chitosan-gelatin coating on the quality of shrimp (Litopenaeus vannamei) under refrigerated condition. Food Control 67: 163-170.
Fernández‐López J, Sevilla L, Sayas‐Barberá E, Navarro C, Marin F and Pérez‐Alvarez J, 2003. Evaluation of the antioxidant potential of hyssop (Hyssopus officinalis L.) and rosemary (Rosmarinus officinalis L.) extracts in cooked pork meat. Journal of Food Science 68(2): 660-664.
Fraternale D, Ricci D, Epifano F and Curini M, 2004. Composition and antifungal activity of two essential oils of hyssop (Hyssopus officinalis L.). Journal of Essential Oil Research 16 (6): 617-622.
Gahruie H , Ziaee E, Eskandari MH and Hosseini SMH, 2017. Characterization of basil seed gum-based edible films incorporated with Zataria multiflora essential oil nanoemulsion. Carbohydrate Polymers 166: 93-103.
Ghahramani-Chermahini A and Sedaghat N, 2022. Evaluation of edible film and modified atmosphere packaging on the improvement of quality and shelf life of bell pepper. Journal of Food Research 32 (4):121-147.
Haghshenas M, Hosseini H, Nayebzadeh K, Kakesh BS, Mahmoudzadeh M, and Fonood RK, 2015. Effect of beta glucan and carboxymethyl cellulose on lipid oxidation and fatty acid composition of pre-cooked shrimp nugget during storage. LWT-Food Science and Technology 62 (2): 1192-1197.
IDF 1991, International IDF Standards.
Jafari SM, Assadpoor E, He Y and Bhandari B, 2008. Re-coalescence of emulsion droplets during high-energy emulsification. Food Hydrocolloids 22 (7): 1191-1202.
Joerger RD, 2007. Antimicrobial films for food applications: a quantitative analysis of their effectiveness. Packaging Technology and Science: An International Journal 20 (4): 231-273.
Keykhosravy K, Khanzadi S, Hashemi M and Azizzadeh M, 2020. Chitosan-loaded nanoemulsion containing Zataria Multiflora Boiss and Bunium persicum Boiss essential oils as edible coatings: Its impact on microbial quality of turkey meat and fate of inoculated pathogens. International Journal of Biological Macromolecules 150: 904-913.
khanzadi S, Hashemi m and Keykhosravy k, 2019. Antimicrobial and antioxidant efficiency of nano emulsion-based edible coating containing ginger (Zingiber officinale) essential oil and its effect on safety and quality attributes of chicken breast fillets. Food Control 106:1-2.
Khanzadi S, Keykhosravy K, Hashemi M and Azizzadeh M, 2020. Alginate coarse/nanoemulsions containing Zataria multiflora Boiss essential oil as edible coatings and the impact on microbial quality of trout fillet. Aquaculture Research 51: 873-881.
Latscha T,  1989. The role of astaxanthin in shrimp pigmentation. Advances in Tropical Aquaculture, Workshop at Tahiti, French Polynesia, 20: 25-29.
Lin D and Zhao Y, 2007. Innovations in the development and application of edible coatings for fresh and minimally processed fruits and vegetables. Comprehensive Reviews In Food Science and Food Safety 6(3): 60-75.
Martinez-Alvarez O, Lopez-Caballero M. E, Montero P and del Carmen Gomez-Guillen M, 2020. The effect of different melanosis-inhibiting blends on the quality of frozen deep-water rose shrimp (Parapenaeus longirostris). Food Control 889: 106-109.
Mendes J, Martins H, Otoni C, Santana N, Silva R, Da Silva A, Silva M, Correia M, Machado G and Pinheiro A, 2018. Chemical composition and antibacterial activity of Eugenia brejoensis essential oil nanoemulsions against Pseudomonas fluorescens. LWT- Food Science and Technology 93: 659-664.
Moghimi R, Ghaderi L, Rafati H, Aliahmadi A and McClements DJ, 2016. Superior antibacterial activity of nanoemulsion of Thymus daenensis essential oil against E. coli. Food Chemistry 194: 410-415.
Najafpour NM, 2001. Compound recognition in essentisl oil of  Hyssopus Officinalis. Iranian Journal of Medicinal and Aromatic Plants Research 12: 78-89.
Nasiri E, Moosavi-Nasab M, Shekarforoush S and Golmakani M, 2014. The effects of Zataria multiflora on inhibition of polyphenoloxidase and melanosis formation in shrimp Litopenaeus vannamei. Iranian Fisheries Scienese Researsch Institue 23 (3): 109-118.
Nirmal NP, 2011. Inhibition of polyphenoloxidase and melanosis in pacific white shrimp (Litopenaeus vannamei) by phenolic compounds. Prince of Songkla University 1-8.
Noori S, Zeynali F and Almasi H, 2018 .Antimicrobial and antioxidant efficiency of nanoemulsion-based edible coating containing ginger (Zingiber officinale) essential oil and its effect on safety and quality attributes of chicken breast fillets. Food Control 84: 312-320.
Noshad M, Alizadeh Behbahani B, Jooyandeh H, Rahmati-Joneidabad M, Ghodsi Sheikhjan M,
Ghorani R and Ebrahimi Hemmati Kaykh M, 2022. The use of okra gum-peppermint essential oil bioactive edible coating to improve shelf-life of buffalo meat. Journal of Food Research 32 (4):13-36.
Nouri M, Khodaiyan F, Razavi S. H and Mousavi MA, 2016. The effect of different chemical and physical processing on the physicochemical and functional characterization of chitosan extracted from shrimp waste species of indian white shrimp. Progress in Rubber Plastics and Recycling Technology 32(1): 39-54.
Ostertag F, Weiss J and McClements DJ, 2012. Low-energy formation of edible nanoemulsions: factors influencing droplet size produced by emulsion phase inversion. Journal of Colloid and Interface Science 388 (1): 95-102.
Ozogul Y, Yuvk, İ, Ucar Y, Durmus M, Kösker A. R, Öz M and Ozogul F, 2017. Evaluation of effects of nanoemulsion based on herb essential oils (rosemary, laurel, thyme and sage) on sensory, chemical and microbiological quality of rainbow trout (Oncorhynchus mykiss) fillets during ice storage. LWT- Food Science and Technology 75: 677-684.
Pirnia M, Tabatabaee Yazdi F, Mortazavi S. A, Mohebbi M, 2020. Comparison and survey of chemical composition and antimicrobial effect Hyssopus officinalis and Frankincense (Boswellia carteri) oils against some of food infectious and spoiling microorganisms In Vitro. Food Science and Technology 17: 91-99.
Qian C and McClements DJ, 2011. Formation of nanoemulsions stabilized by model food-grade emulsifiers using high-pressure homogenization: factors affecting particle size. Food Hydrocolloids 25(5): 1000-1008.
Rabea E. I, Badawy M. E.-T, Stevens C. V, Smagghe G and Steurbaut W, 2003. Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules 4(6): 1457-1465.
Salehi S, Khodanazary A and Zamani E, 2018. Comparative changes in peeled white shrimp (Metapenaeus affinis) during ice and refrigerator storage. Iranian Scientific Fisheries Journal 27(4): 123-136.
Salvia-Trujillo L, Rojas-Graü M. A, Soliva-Fortuny R and Martín-Belloso O, 2013. Effect of processing parameters on physicochemical characteristics of microfluidized lemongrass essential oil-alginate nanoemulsions. Food Hydrocolloids 30 (1): 401-407.
Sani MA, Ehsani A and Hashemi M, 2017. Whey protein isolate/cellulose nanofibre/TiO2 nanoparticle/rosemary essential oil nanocomposite film: Its effect on microbial and sensory quality of lamb meat and growth of common foodborne pathogenic bacteria during refrigeration. International Journal of Food Microbiology 251: 8-14.
Shantha NC and Decker EA, 1994. Rapid, sensitive, iron-based spectrophotometric methods for determination of perorlride values of food lipids. Journal of AOAC International 77(2): 421-424.
Taheri T, Fazlara A, Roomiani L and Taheri S, 2018. Effect of chitosan coating enriched with cumin (cuminum cyminum l.) essential oil on the quality of refrigerated turkey breast meat. Italian Journal of Food Science 30 (3): 628-640.
Wang Q, Lei J, Ma J, Yuan G and Sun H, 2018. Effect of chitosan-carvacrol coating on the quality of Pacific white shrimp during iced storage as affected by caprylic acid. International Journal of Biological Macromolecules 106: 123-129.
Yuan G, Lv H, Tang W, Zhang X and Sun H, 2016. Effect of chitosan coating combined with pomegranate peel extract on the quality of Pacific white shrimp during iced storage. Food Control 59: 818-823.
Yuan G, Zhang X, Tang W and Sun H, 2016. Effect of chitosan coating combined with green tea extract on the melanosis and quality of Pacific white shrimp during storage in ice. CyTA-Journal of Food 14 (1): 35-40.