بررسی خواص فیزیکوشیمیایی و میکروبی فیلم نانوکامپوزیت کیتوزان/نقره/عصاره زیره سبز (L. Cuminum cyminum)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه ازاد دامغان

2 استادیار علوم و صنایع غذایی دانشکده کشاورزی دانشگاه آزاد اسلامی دامغان

10.22034/fr.2021.36380.1701

چکیده

زمینه مطالعاتی: پلی‌فنل‌ها با خواص آنتی‌اکسیدانی مسئول خصوصیات سلامت بخش و ضدمیکروبی زیره هستند. روش کار: در این مطالعه خواص فیزیکوشیمیایی و میکروبی نانو کامپوزیتهای نقره (Ag)، کیتوزان (Ch) حاوی عصاره زیره سبز (E) مورد بررسی قرار گرفت. در ابتدا عصاره زیره سبز با روش‌های استخراج عصاره فراصوت و ماسراسیون و حلال آب/اتانول استخراج شد. عصاره حاصل تحت آزمون‌های تعیین فنول کل، توکوفرول قرار گرفت. در ادامه فیلم نانوکامپوزیت کیتوزان/نقره (15/0مول/لیتر) با سه غلظت 2/0، 4/0 و 1 درصد عصاره زیره سبز تهیه و تحت آزمون‌های فیزیکوشیمیایی (اندازهگیری ضخامت، نرخ عبور بخار آب، حلالیت فیلم، مقاومت کششی، میکروسکوپ الکترونی روبشی (SEM) و افزایش طول فیلم در لحظه پاره شدن) و میکروبی (قطر عدم هاله رشد) قرار گرفت. نتایج: نتایج نشان داد که عصاره زیره سبز استخراج شده به روش ماسراسیون دارای ترکیبات فنولی (18/74 میلی‌گرم گالیک‌اسید/گرم وزن خشک) و توکوفرولی (05/24 میلی‌گرم آلفاتوکوفرول/گرم وزن خشک) بالایی میباشد. نتایج آزمایشات حاکی از آن بود با افزایش عصاره زیره سبز تا یک درصد ضخامت فیلم (093/0 میلیمتر)، نرخ عبور بخار آب (g/s.m2 81/3، حلالیت فیلم (45/26 %) و افزایش طول فیلم در لحظه پاره شدن (21/30 %) افزایش معنی‌داری را نشان داد (p <0.05)، ولی مقاومت کششی فیلم (10/16 مگاپاسکال) با افزایش غلظت کاهش یافت. نتایج حاصل از آزمون میکروبی نشان داد که با افزایش غلظت عصاره زیره سبز تا یک درصد، خاصیت ضدمیکروبی فیلم نانوکامپوزیت کیتوزان/نقره افزایش یافت، نتایج میکروبی نشان داد فیلم نانوکامپوزیت کیتوزان/نقره حاوی عصاره زیره سبز به ترتیب دارای خاصیت ضدمیکروبی علیه استافیلوکوکوس آورئوس> آسپرژیلوس نایجر> کاندیدا آلبیکانس> اشرشیا کلی بوده است. نتیجه گیری نهایی: بطور کلی این مطالعه نشان داد فیلم تهیه شده ویژگی کیفی مناسبی را جهت بست بندی مواد غذایی دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of Physicochemical and Microbial Properties of Chitosan / Silver / Cumin Extract Nanocomposite Film (L. Cuminum cyminum)

نویسندگان [English]

  • zahra khosroabadi 1
  • lila nori 2
1 Free University of Damghan
2 Assistant Professor of Food Science and Technology, Faculty of Agriculture, Islamic Azad University, Damghan
چکیده [English]

Background: Polyphenols with antioxidant properties are responsible for the health and antimicrobial properties of L. Cuminum cyminum. Materials and Methods: In this study, the physicochemical and microbial properties of silver (Ag), chitosan (Ch) containing L. Cuminum cyminum extract (E) were investigated. L. Cuminum cyminum extract was first extracted by ultrasonic extraction and water / ethanol solvent extraction methods. The extract was subjected to total phenol tests, tocopherol. In the following, chitosan / silver nanocomposite film (0.15 mol/ l) was prepared at three concentrations of 0.2, 0.4 and 1% of cumin extract and subjected to physicochemical tests (thickness measurement, water vapor passage rate, Scanning Electron Microscope (SEM), film solubility, resistance). Tensile and film length increase at the moment of tear) and microbial (no growth zone diameter). Results: The results showed that the extract of L. Cuminum cyminum extracted by massage method had high phenolic compounds (74.18 mg / g dry weight) and tocopherol (24.05 mg alpha-tocopherol / g dry weight). Experimental results showed that by increasing L. Cuminum cyminum extract to 1% film thickness (0.093 mm), water vapor passage rate (3.81 g / s.m2, film solubility (26.45%) and increasing film length in The tearing moment (30.21%) showed a significant increase (P <0.05), but the tensile strength of the film (16.10 MPa) decreased with increasing concentration.The results of microbial test showed that with increasing the extract concentration L. Cuminum cyminum increased by 1% the antimicrobial activity of chitosan / silver nanocomposite film, microbial results showed that the chitosan / silver nanocomposite film containing antiseptic extract had antimicrobial activity against Staphylococcus aureus> Aspergus, respectively. Los niger> Candida albicans> E. coli, respectively. Conclusions: This study showed generally good quality feature film for food packaging is closed.Background: Polyphenols with antioxidant properties are responsible for the health and antimicrobial properties of L. Cuminum cyminum. Materials and Methods: In this study, the physicochemical and microbial properties of silver (Ag), chitosan (Ch) containing L. Cuminum cyminum extract (E) were investigated. L. Cuminum cyminum extract was first extracted by ultrasonic extraction and water / ethanol solvent extraction methods. The extract was subjected to total phenol tests, tocopherol. In the following, chitosan / silver nanocomposite film (0.15 mol/ l) was prepared at three concentrations of 0.2, 0.4 and 1% of cumin extract and subjected to physicochemical tests (thickness measurement, water vapor passage rate, Scanning Electron Microscope (SEM), film solubility, resistance). Tensile and film length increase at the moment of tear) and microbial (no growth zone diameter). Results: The results showed that the extract of L. Cuminum cyminum extracted by massage method had high phenolic compounds (74.18 mg / g dry weight) and tocopherol (24.05 mg alpha-tocopherol / g dry weight). Experimental results showed that by increasing L. Cuminum cyminum extract to 1% film thickness (0.093 mm), water vapor passage rate (3.81 g / s.m2, film solubility (26.45%) and increasing film length in The tearing moment (30.21%) showed a significant increase (P <0.05), but the tensile strength of the film (16.10 MPa) decreased with increasing concentration.The results of microbial test showed that with increasing the extract concentration L. Cuminum cyminum increased by 1% the antimicrobial activity of chitosan / silver nanocomposite film, microbial results showed that the chitosan / silver nanocomposite film containing antiseptic extract had antimicrobial activity against Staphylococcus aureus> Aspergus, respectively. Los niger> Candida albicans> E. coli, respectively. Conclusions: This study showed generally good quality feature film for food packaging is closed.Background: Polyphenols with antioxidant properties are responsible for the health and antimicrobial properties of L. Cuminum cyminum. Materials and Methods: In this study, the physicochemical and microbial properties of silver (Ag), chitosan (Ch) containing L. Cuminum cyminum extract (E) were investigated. L. Cuminum cyminum extract was first extracted by ultrasonic extraction and water / ethanol solvent extraction methods. The extract was subjected to total phenol tests, tocopherol. In the following, chitosan / silver nanocomposite film (0.15 mol/ l) was prepared at three concentrations of 0.2, 0.4 and 1% of cumin extract and subjected to physicochemical tests (thickness measurement, water vapor passage rate, Scanning Electron Microscope (SEM), film solubility, resistance). Tensile and film length increase at the moment of tear) and microbial (no growth zone diameter). Results: The results showed that the extract of L. Cuminum cyminum extracted by massage method had high phenolic compounds (74.18 mg / g dry weight) and tocopherol (24.05 mg alpha-tocopherol / g dry weight). Experimental results showed that by increasing L. Cuminum cyminum extract to 1% film thickness (0.093 mm), water vapor passage rate (3.81 g / s.m2, film solubility (26.45%) and increasing film length in The tearing moment (30.21%) showed a significant increase (P <0.05), but the tensile strength of the film (16.10 MPa) decreased with increasing concentration.The results of microbial test showed that with increasing the extract concentration L. Cuminum cyminum increased by 1% the antimicrobial activity of chitosan / silver nanocomposite film, microbial results showed that the chitosan / silver nanocomposite film containing antiseptic extract had antimicrobial activity against Staphylococcus aureus> Aspergus, respectively. Los niger> Candida albicans> E. coli, respectively. Conclusions: This study showed generally good quality feature film for food packaging is closed.Background: Polyphenols with antioxidant properties are responsible for the health and antimicrobial properties of L. Cuminum cyminum. Materials and Methods: In this study, the physicochemical and microbial properties of silver (Ag), chitosan (Ch) containing L. Cuminum cyminum extract (E) were investigated. L. Cuminum cyminum extract was first extracted by ultrasonic extraction and water / ethanol solvent extraction methods. The extract was subjected to total phenol tests, tocopherol. In the following, chitosan / silver nanocomposite film (0.15 mol/ l) was prepared at three concentrations of 0.2, 0.4 and 1% of cumin extract and subjected to physicochemical tests (thickness measurement, water vapor passage rate, Scanning Electron Microscope (SEM), film solubility, resistance). Tensile and film length increase at the moment of tear) and microbial (no growth zone diameter). Results: The results showed that the extract of L. Cuminum cyminum extracted by massage method had high phenolic compounds (74.18 mg / g dry weight) and tocopherol (24.05 mg alpha-tocopherol / g dry weight). Experimental results showed that by increasing L. Cuminum cyminum extract to 1% film thickness (0.093 mm), water vapor passage rate (3.81 g / s.m2, film solubility (26.45%) and increasing film length in The tearing moment (30.21%) showed a significant increase (P <0.05), but the tensile strength of the film (16.10 MPa) decreased with increasing concentration.The results of microbial test showed that with increasing the extract concentration L. Cuminum cyminum increased by 1% the antimicrobial activity of chitosan / silver nanocomposite film, microbial results showed that the chitosan / silver nanocomposite film containing antiseptic extract had antimicrobial activity against Staphylococcus aureus> Aspergus, respectively. Los niger> Candida albicans> E. coli, respectively. Conclusions: This study showed generally good quality feature film for food packaging is closed.

کلیدواژه‌ها [English]

  • Antioxidant
  • Edible coating
  • Cumin
  • Chitosan / Ag nanocomposite
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